Anti pgc1α

Manufactured by GeneTex

Anti-PGC1α is a primary antibody that recognizes the Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha (PGC1α) protein. PGC1α is a transcriptional coactivator that regulates genes involved in energy metabolism. This antibody can be used for applications such as Western blotting and immunohistochemistry to detect and analyze PGC1α expression.

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2 protocols using anti pgc1α

Protein Quantification and Western Blot Analysis

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To assess protein level in brain or cells, lysates were prepared in SDS lysis buffer containing protease inhibitors and phosphatase inhibitors. The amount of proteins in lysates were normalized by BCA protein assay kit (Millipore). Proteins were resolved by SDS–polyacrylamide gel electrophoresis and then transferred to nitrocellulose membranes (PerkinElmer). Transferred blots were incubated with primary antibodies, anti-PGAM5 (1:500, Santa Cruz, SC-515880), anti-GAPDH (1:5000, Genetex, GTX100118), anti-TUJ1 (1:5000, BioLegend, #801202), anti-GFAP (1:5000, Genetex, GTX108711), anti-PGC1α (1:500, Genetex, GTX37356), anti-NRF1 (1:1000, Genetex, GTX103179), anti-TFAM (1:500, Genetex, GTX59889), or anti-TIM23 (1:500, Santa Cruz, SC-514463) overnight. Then the blots were incubated with secondary antibodies, IRDye 800CW goat anti-rabbit IgG secondary antibody (1:1000, LI-COR, # 926-32211) or goat anti-mouse IgG secondary antibody Alexa Fluor™ 700 (1:1000, Invitrogen, # A-21036), for 1 h. Membranes were imaged by ChemiDoc™ MP Imaging System (Bio-Rad) and the signal intensity of bands was quantified by Image Lab software (Bio-Rad, version 6.1.0) (Additional file 2).

Liang M.Z., Lu T.H, & Chen L. (2023). Timely expression of PGAM5 and its cleavage control mitochondrial homeostasis during neurite re-growth after traumatic brain injury. Cell & Bioscience, 13, 96.

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Immunoblotting and Immunoprecipitation Protocols

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Western analyses and immunoprecipitations were conducted as described (3 (link), 4 (link), 16 (link), 17 (link), 19 (link), 20 (link), 27 (link)) using: anti-phospho-serine-473-Akt, anti-glyceraldehyde-phosphate dehydrogenase (GAPDH), anti-WD40/ProF, anti-PEPCK, anti-G6Pase, anti-FAS, and anti-aPKC (Santa Cruz Biotechnologies, Santa Cruz, CA); antiphospho-threonine-560/555-PKC-ζ/λ/ι (Invitrogen, Carlsbad, CA); anti-p-serine-256-FoxO1, anti-FoxO1 (Abnova, Walnut, CA); anti-PKC-λ/ι (Transduction Antibodies, Bedford, MA); anti-phospho-serine-9-GSK3β, anti-GSK3β, anti-phospho-serine-2248-mTOR, anti-mTOR, anti-ACC and mouse anti-Akt Mab (Cell Signaling Technologies, Danvers, MA); anti-IRS-1, anti-IRS-2 (Millipore, Temecula, CA); anti-SREBP (Thermo Fisher Scientific, Freemont, CA); anti-PKC-ζ (Dr. Todd C. Sacktor, State University of New York, NY); and anti-PGC-1α (Genetex, Irvine, CA), a C-terminally-directed antiserum that measures 77kDa and 91kDa PGC-1α isoforms; however, we report only on the much more abundant 77kDa isoform, which, is specifically present in human liver, and strongly influenced by FoxO1 (11 (link)).

Sajan M.P., Ivey RA I.I.I, & Farese R.V. (2015). BMI-Related Progression of Atypical PKC-dependent Aberrations in Insulin Signaling Through IRS-1, Akt, FoxO1 and PGC-1α in Livers of Obese and Type 2 Diabetic Humans. Metabolism: clinical and experimental, 64(11), 1454-1465.

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