A modified Histidine-Tryptophan-Ketoglutarate (HTK) organ preservation solution was prepared in house with 5% (w/v) polyethylene glycol (PEG), average M.W. 8000, added as an oncotic agent to minimize cellular edema [6 (link)–9 (link)]. The modified HTK-PEG solution was sterilized by filtration through a 0.22 μm filter unit, and the pH and density was measured. The density of the HTK-PEG preservation solution was measured using a Densito 30PX densitometer (Mettler Toledo) approximately 1.022 g/mL at 20 °C. The solution pH was 7.2-7.4 at 4 °C with a theoretical osmolality of approximately 315 mOsm/kg.
Densito 30 px densitometer
Manufactured by Mettler Toledo
Sourced in Switzerland
The Densito 30 PX is a densitometer by Mettler Toledo. It is a portable and robust instrument designed to measure the density of liquids. The device features automatic temperature compensation and provides density measurements with high accuracy.
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Lab products found in correlation
4 protocols using densito 30 px densitometer
1
Optimized Organ Preservation Solution
2
Fermentation Process Dynamics by CO2 Emission
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Dynamics of the fermentation process were controlled based on CO2 emission in time. To this end, the fermentation samples were weighed on a WTB 2000 scale by RADWAG company (Radom, Poland) every 24 h throughout the alcoholic fermentation. Extract content was determined with a Densito 30 PX densitometer by Mettler-Toledo company (Greifensee, Switzerland). The samples were earlier centrifuged using an MPW-351R laboratory centrifuge (2675 centrifugal force (RCF), 6000× g, 10 min) by MPW MED. INSTRUMENTS company (Warszawa, Poland). Extract content was measured in the resultant supernatant at a temperature of 20 °C. The pH value was measured with an MP 220 pH-meter by Mettler Toledo company (Greifensee, Switzerland).
3
Yeast Fermentation Analysis Techniques
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Fermentations were carried out in an incubator from Selecta (Barcelona, Spain) using Saccaromyces cerevisiae cells from Lallemand Inc (Montreal, Quebec, Canada).
For density measurements, a Densito 30 PX densitometer from Mettler-Toledo GmbH (Greifensee, Switzerland) was employed. Musts and wine composition was analysed with a Surveyor Plus HPLC chromatography system from Thermo Fisher Scientific (Waltham, MA, USA) equipped with refraction index and UV-vis detectors. A Hyper REZ XP carbohydrate H+8 column, also from Thermo Fisher Scientific, was employed as stationary phase at 50 °C. Samples were filtered with 0.2 µm nylon filter devices.
A series of buffers and solutions were employed. Coating buffer: 50 mM carbonate-bicarbonate buffer, pH 9.6; PBS: 10 mM phosphate buffer, pH 7.4, with 140 mM NaCl; PBST: PBS containing 0.05% (v/v) Tween 20; PB: 100 mM sodium phosphate buffer, pH 7.4; washing solution: 150 mM NaCl containing 0.05% (v/v) Tween 20; enzyme substrate buffer: 25 mM citrate and 62 mM sodium phosphate buffer, pH 5.4.
For density measurements, a Densito 30 PX densitometer from Mettler-Toledo GmbH (Greifensee, Switzerland) was employed. Musts and wine composition was analysed with a Surveyor Plus HPLC chromatography system from Thermo Fisher Scientific (Waltham, MA, USA) equipped with refraction index and UV-vis detectors. A Hyper REZ XP carbohydrate H+8 column, also from Thermo Fisher Scientific, was employed as stationary phase at 50 °C. Samples were filtered with 0.2 µm nylon filter devices.
A series of buffers and solutions were employed. Coating buffer: 50 mM carbonate-bicarbonate buffer, pH 9.6; PBS: 10 mM phosphate buffer, pH 7.4, with 140 mM NaCl; PBST: PBS containing 0.05% (v/v) Tween 20; PB: 100 mM sodium phosphate buffer, pH 7.4; washing solution: 150 mM NaCl containing 0.05% (v/v) Tween 20; enzyme substrate buffer: 25 mM citrate and 62 mM sodium phosphate buffer, pH 5.4.
4
Yeast Fermentation Kinetics Analysis
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Fermentations were carried out in laboratory-scale fermenters using 100-mL bottles filled with 60 mL of SM, which were fitted with closures that enabled carbon dioxide to escape and samples to be removed, at 28ºC and 12ºC, with continuous orbital shaking at 100 rpm. Yeast cell growth was determined by absorbance at 600 nm and by plating adequate dilutions on YPD agar by the end of fermentation. Plates were incubated for 2 days at 30ºC. Fermentation was monitored by measuring the density of the media (g/L) in a Densito 30 PX densitometer (Mettler Toledo, Switzerland).
Fermentation was considered completed when density was below 998 g/L. The kinetics of these fermentations was estimated by calculating the time needed to ferment 5% (T5), 50% (T50) and 100% (T100) of sugars in SM by representing density reduction vs. time and adjusting these values to the four-parameters logistic equation proposed by Speers et al. (2003) . T5, T50 and T100 approximately matched the beginning (lag phase), middle (end of the exponential phase) and end of fermentation, respectively.
Fermentation was considered completed when density was below 998 g/L. The kinetics of these fermentations was estimated by calculating the time needed to ferment 5% (T5), 50% (T50) and 100% (T100) of sugars in SM by representing density reduction vs. time and adjusting these values to the four-parameters logistic equation proposed by Speers et al. (2003) . T5, T50 and T100 approximately matched the beginning (lag phase), middle (end of the exponential phase) and end of fermentation, respectively.
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