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6130 mass spectrometer

Manufactured by Agilent Technologies
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The 6130 mass spectrometer is a laboratory instrument designed for the analysis of chemical compounds. It is capable of detecting and identifying various molecules based on their mass-to-charge ratio. The core function of the 6130 mass spectrometer is to provide accurate and reliable data for analytical applications.

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Lab products found in correlation

400 mhz spectrometer, by Agilent Technologies (3 mentions) 6210 time of flight lc ms system, by Agilent Technologies (3 mentions) Masshunter software version b 02, by Agilent Technologies (2 mentions) 1200 hplc, by Agilent Technologies (1 mentions) Diode array detector, by Agilent Technologies (1 mentions) Luna c18 column, by Phenomenex (1 mentions) 1260 hplc system, by Agilent Technologies (1 mentions) Sx20 stopped flow spectrometer, by Applied Photophysics (1 mentions) S500 spectrometer, by Agilent Technologies (1 mentions) Flash ea 1112 elemental analyser, by Thermo Fisher Scientific (1 mentions) Nicolet is50 ftir spectrometer, by Thermo Fisher Scientific (1 mentions)

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6130 quadrupole mass spectrometer (27 citations) 6130 single quadrupole mass spectrometer (14 citations) Spectrometers (10 citations) Agilent 6130 single quadrupole mass spectrometer (6 citations) 6130 single quad mass spectrometer (4 citations) 6130 Series ESI mass spectrometer (3 citations)

5 protocols using 6130 mass spectrometer

1

Analytical Characterization of Chemical Compounds

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Compounds were synthesized in the National Center for Advancing Translational Sciences (Supporting Information Table S2). Purity determination was performed using an Agilent diode array detector for both method 1 and method 2 (below). Mass determination was performed using an Agilent 6130 mass spectrometer with electrospray ionization in the positive mode. Chiral separations were performed by normal phase chromatography using a CHIRALPAKAD column (5 cm × 50 cm, 20 μm) on an Agilent 1200 HPLC. 1H NMR spectra were recorded on Varian 400 MHz spectrometers. Chemical shifts are reported in ppm with undeuterated solvent (DMSO-d6 at 2.49 ppm) as internal standard for DMSO-d6 solutions. All of the analogs tested in the biological assays have purity greater than 95%, based on both analytical methods. High resolution mass spectrometry was recorded on Agilent 6210 time-of-flight LC/MS system. Confirmation of molecular formula was accomplished using electrospray ionization in the positive mode with the Agilent Masshunter software (version B.02).
Horton J.R., Liu X., Wu L., Zhang K., Shanks J., Zhang X., Rai G., Mott B.T., Jansen D.J., Kales S.C., Henderson M.J., Pohida K., Fang Y., Hu X., Jadhav A., Maloney D.J., Hall M.D., Simeonov A., Fu H., Vertino P.M., Yan Q, & Cheng X. (2018). Insights into the Action of Inhibitor Enantiomers against Histone Lysine Demethylase 5A. Journal of medicinal chemistry, 61(7), 3193-3208.
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2

HPLC-MS Analysis of Organic Analogs

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Analysis was performed on an Agilent 1260 with a 7 min gradient from 4% to 100% acetonitrile (containing 0.025% trifluoroacetic acid) in water (containing 0.05% trifluoroacetic acid) over 8 min run time at a flow rate of 1 mL/min. A Phenomenex Luna C18 column (3 µm, 3 mm × 75 mm) was used at a temperature of 50 °C. Purity determination was performed using an Agilent diode array detector for both method 1 and method 2. Mass determination was performed using an Agilent 6130 mass spectrometer with electrospray ionization in the positive mode. All of the analogs for assay have purity greater than 95% based on both analytical methods. 1H NMR spectra were recorded on Varian 400 MHz spectrometers. High resolution mass spectrometry results were recorded on Agilent 6210 time-of-flight LC/MS system.
Yang S.M., Yasgar A., Miller B., Lal-Nag M., Brimacombe K., Hu X., Sun H., Wang A., Xu X., Nguyen K., Oppermann U., Ferrer M., Vasiliou V., Simeonov A., Jadhav A, & Maloney D.J. (2015). Discovery of NCT-501, a Potent and Selective Theophylline-Based Inhibitor of Aldehyde Dehydrogenase 1A1 (ALDH1A1). Journal of medicinal chemistry, 58(15), 5967-5978.
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3

ESI-TOF-MS Analysis of Amyloid-Beta Complexes

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ESI-TOF-MS experiments were performed on an Agilent 6130 mass spectrometer connected to an Agilent 1260 HPLC system. Samples were analyzed by direct infusion (1-4 μL) of analyte into a mobile phase of 1:1 water:acetonitrile containing 5 mM ammonium acetate (pH unmodified), flowing at 0.3 mL/min and maintained at 30°C. All components of the mobile phase were mass spectrometry grade. Nitrogen drying gas was heated to 250°C, and run at 5 L/min with a nebulizing pressure of 15 psig. Voltages were: capillary 3 kV, fragmentor 175 V, skimmer 30 V, octopole 250 V. Samples were prepared as ~4 mg/mL of total protein (Aβ1−16) in ammonium carbonate (0.02 M, pH 9) buffer with 0 or 1 equivalents of the Ru-N complexes.
Gomes L.M., Bataglioli J.C., Jussila A.J., Smith J.R., Walsby C.J, & Storr T. (2019). Modification of Aβ Peptide Aggregation via Covalent Binding of a Series of Ru(III) Complexes. Frontiers in Chemistry, 7, 838.
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4

Characterization of Novel Organic Compounds

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All reagents and solvents were obtained from commercial sources and used without further purification. All aqueous solutions were prepared using either deionized or distilled water. 1H-NMR (500 MHz) spectra were recorded on the Varian S500 spectrometer. Elemental analyses were performed using the Thermo Scientific FlashEA 1112 elemental analyser. Mass spectra were obtained using the Agilent 6130 mass spectrometer. Fourier-transform infrared (FT-IR) spectra were recorded on the Thermo Scientific Nicolet iS50 FT-IR spectrometer. Potentiometric measurements were carried out in the pKa mode using the Metrohm 808 Titrando titrator with the Tiamo 2.3 software and Pt1000 pH electrode. Kinetic studies were carried out in the monochromator mode using the Applied Photophysics SX20 stopped-flow spectrometer equipped with a thermoelectric temperature controller (±0.5°C).
Park D, & Lee M.S. (2019). Kinetic study of catalytic CO2 hydration by metal-substituted biomimetic carbonic anhydrase model complexes. Royal Society Open Science, 6(8), 190407.
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5

Analytical Characterization of Synthetic Compounds

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Compounds were synthesized at the National Center for Advancing Translational Sciences. Purity determination was performed using an Agilent Diode Array Detector for both Method 1 and Method 2 (below). Mass determination was performed using an Agilent 6130 mass spectrometer with electrospray ionization in the positive mode. 1H NMR spectra were recorded on Varian 400 MHz spectrometers. Chemical shifts for final compounds are reported in ppm with undeuterated solvent (DMSO-d6 at 2.50 ppm) as internal standard for DMSO-d6 solutions. Chemical shifts for intermediate compounds are reported in ppm with undeuterated solvent (CDCl3 at 7.26 ppm) as internal standard for CDCl3 solutions. All the analogs tested in the biological assays have purity greater than 95%, based on both analytical methods. High resolution mass spectrometry was recorded on Agilent 6210 Time-of-Flight LC/MS system. Confirmation of molecular formula was accomplished using electrospray ionization in the positive mode with the Agilent Masshunter software (version B.02).
Horton J.R., Woodcock C.B., Chen Q., Liu X., Zhang X., Shanks J., Rai G., Mott B.T., Jansen D.J., Kales S.C., Henderson M.J., Cyr M., Pohida K., Hu X., Shah P., Xu X., Jadhav A., Maloney D.J., Hall M.D., Simeonov A., Fu H., Vertino P.M, & Cheng X. (2018). Structure-Based Engineering of Irreversible Inhibitors against Histone Lysine Demethylase KDM5A. Journal of medicinal chemistry, 61(23), 10588-10601.
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