The material for the microbiological analyses was collected from the cloaca and cecum once with the use of sterile swabs. LB nutrient broth (BioMaxima, Lublin, Poland) incubated at 37 °C for 24 h was used to multiply the bacteria in the laboratory conditions. Next, the material was transferred to SalmonellaShigella (S-S) agar (BioMaxima, Lublin, Poland) and grown at 37 °C for 24 h. After incubation on a selective medium, bacterial colonies originating from the chickens were selected for further identification and analysis. The entire collection of the isolated strains was analyzed biochemically. Each strain was identified using the commercial API 20E test kit (bioMérieux, Warsaw, Poland).
Salmonellashigella s s agar
Manufactured by Biomaxima
Sourced in Poland
SalmonellaShigella (S-S) agar is a selective and differential culture medium used for the isolation and identification of Salmonella and Shigella species from clinical and food samples. It inhibits the growth of Gram-positive bacteria and many Gram-negative bacteria, while allowing the growth of Salmonella and Shigella.
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Lab products found in correlation
2 protocols using salmonellashigella s s agar
1
Isolation and Identification of Chicken Gut Bacteria
2
Microbial Analysis of Chicken Cecum and Cloaca
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The material for microbiological analysis was collected with sterile swabs once from 5 birds per treatment (one chicken per cage) from the cecum (3 samples per chicken) and from the cloaca (3 samples per chicken). LB nutrient broth (BioMaxima, Lublin, Poland) incubated at 37°C for 24 h was used for laboratory bacterial culture. Next, the material was transferred to Salmonella-Shigella (S-S) agar (BioMaxima, Lublin, Poland) and the culture was grown at 37°C for 24 h. After incubation on a selective medium, bacterial colonies of the experimental isolates were selected for further identification and analysis. All isolated strains were analyzed biochemically. Each strain was tested using the commercial API 20E kit (bioMérieux, Poland).
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