HbA1c was measured from blood samples collected from the mice models using Mouse HbA1c Kit (Catalogue #80310, Crystal Chem, USA), according to the manufacturer's instructions and normalized against total hemoglobin.
Mouse hba1c kit
Manufactured by Crystal Chem
Sourced in United States
The Mouse HbA1c Kit is a laboratory equipment used for the quantitative determination of glycated hemoglobin (HbA1c) levels in mouse blood samples. It provides a measure of average blood glucose levels over a 2-3 month period.
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Lab products found in correlation
2 protocols using mouse hba1c kit
1
Measuring Murine HbA1c Levels
2
HbA1c Measurement in Mouse Blood
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HbA1c was measured using Crystal chem mouse HbA1c kit (cat. no. 80310). Briefly, 5 µL of blood was taken and mixed with lysate buffer to prepare sample lysate. Next, 25 µL of sample lysates were added to 2 wells in a 96-wells polystyrene microplate and mixed with protease buffer A and buffer 1B. The plate was incubated for 5 min at 37 °C and the absorbance was measured at 700 nm. Next, enzyme solution was added and incubated for 3 min at 37 °C and the final absorbance was measured at 700 nm. The difference between the two absorbance measurements was used for calculating HbA1c.
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