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Pe conjugated anti trop 2 antibody

Manufactured by Thermo Fisher Scientific

The PE-conjugated anti-Trop-2 antibody is a laboratory reagent used for the detection and analysis of the Trop-2 protein in biological samples. It consists of a phycoerythrin (PE) fluorescent dye conjugated to an antibody that specifically binds to the Trop-2 protein.

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2 protocols using pe conjugated anti trop 2 antibody

1

Quantification of Trop-2 Expression via Flow Cytometry

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Expression of Trop-2 on the cell surface is based on flow cytometry. Briefly, cells were harvested with Accutase Cell Detachment Solution (Becton Dickinson (BD); Franklin Lakes, NJ; Cat. No. 561527) and assayed for Trop-2 expression using QuantiBRITE PE beads (BD Cat. No. 340495) and a PE-conjugated anti-Trop-2 antibody (eBiosciences, Cat. No. 12-6024) following the manufacturer's instructions. Data were acquired on a FACSCalibur Flow Cytometer (BD) with CellQuest Pro software, with analysis using Flowjo software (Tree Star; Ashland OR).
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2

Quantifying Trop-2 Cell Surface Expression

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Expression of Trop-2 on the cell surface is based on flow cytometry making use of QuantiBRITE PE beads (BD Cat. No. 340495) and a PE-conjugated anti-Trop-2 antibody (eBiosciences, Cat. No. 12-6024), as described previously [26 (link)] and presented in the Supplementary Materials.
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