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Tetramethyl rhodamine b

Manufactured by Merck Group
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Tetramethyl rhodamine B is a fluorescent dye commonly used in biological research applications. It is a rhodamine-based compound that exhibits excitation and emission wavelengths suitable for various microscopy and flow cytometry techniques. The dye can be utilized to label and visualize cellular structures, proteins, or other biomolecules of interest.

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Lab products found in correlation

Anti plk1 pt210, by Abcam (2 mentions) Anti h3 phospho ser10, by Merck Group (2 mentions)

Spelling variants of this product

Rhodamine B (507 citations) Tetramethyl rhodamine B isothiocyanate-conjugated phalloidin (9 citations) Tetramethyl rhodamine B isothiocyanate (5 citations) Phalloidin-tetramethyl rhodamine B isothiocyanate (P1951) (4 citations) Tetramethyl-rhodamine B isothiocyanate-phalloidin (TRITC-phalloidin; (3 citations)

2 protocols using tetramethyl rhodamine b

1

Western Blotting and Immunofluorescence Protocols

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Whole-cell lysates were prepared by lysing the cells in sample buffer. SDS-PAGE and immunoblotting were performed following standard procedures. Anti-α-tubulin antibody (clone B512, T5168, 1:2000) and anti-H3 phospho-Ser10 (06-570, 1:1000) were purchased from Sigma and Upstate Biotech (now Merck-Millipore), respectively, and anti-Plk1 pT210 from Abcam (ab39068, 1:500). Rabbit polyclonal (WCE1186, 1:500) and mouse monoclonal anti-INCENP (3D3, 1:500) were previously described (Cooke et al., 1987 (link); Mackay et al., 1993 (link)). For F-actin staining, phalloidin conjugated to tetramethyl rhodamine B purchased from Sigma was used at a 1:500 dilution. Rabbit anti-PRC1 antibody was raised against full-length His-tagged chicken PRC1 protein and used at 1:500 dilution.
Papini D., Fant X., Ogawa H., Desban N., Samejima K., Feizbakhsh O., Askin B., Ly T., Earnshaw W.C, & Ruchaud S. (2019). Cell cycle-independent furrowing triggered by phosphomimetic mutations of the INCENP STD motif requires Plk1. Journal of Cell Science, 132(21), jcs234401.
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2

Western Blotting and Immunofluorescence Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-cell lysates were prepared by lysing the cells in sample buffer. SDS-PAGE and immunoblotting were performed following standard procedures. Anti-α-tubulin antibody (clone B512, T5168, 1:2000) and anti-H3 phospho-Ser10 (06-570, 1:1000) were purchased from Sigma and Upstate Biotech (now Merck-Millipore), respectively, and anti-Plk1 pT210 from Abcam (ab39068, 1:500). Rabbit polyclonal (WCE1186, 1:500) and mouse monoclonal anti-INCENP (3D3, 1:500) were previously described (Cooke et al., 1987 (link); Mackay et al., 1993 (link)). For F-actin staining, phalloidin conjugated to tetramethyl rhodamine B purchased from Sigma was used at a 1:500 dilution. Rabbit anti-PRC1 antibody was raised against full-length His-tagged chicken PRC1 protein and used at 1:500 dilution.
Papini D., Fant X., Ogawa H., Desban N., Samejima K., Feizbakhsh O., Askin B., Ly T., Earnshaw W.C, & Ruchaud S. (2019). Cell cycle-independent furrowing triggered by phosphomimetic mutations of the INCENP STD motif requires Plk1. Journal of Cell Science, 132(21), jcs234401.
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