Theophylline (1,3-dimethylxanthine), caffeine (1,3,7-trimethylxanthine), isobutylmethylxanthine (3-isobutyl-1-methylxanthine, IBMX), histamine, testosterone (4-androstene-17β-ol-3-one, TES), and flutamide were obtained from Sigma Chem. Co. (St. Louis, MO, USA). Notably, 4-Aminopyridine (4-AP) was purchased from Research Chemical LTD (Word Hill, MA, USA), and iberiotoxin was acquired from Santa Cruz Biotechnology (Dallas, TX, USA). TES and flutamide were diluted in absolute ethanol and IBMX in DMSO. The highest percentage used was 0.1% v/v of the vehicle.
Iberiotoxin
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Iberiotoxin is a highly potent and selective blocker of large-conductance calcium-activated potassium (BK) channels. It is a peptide toxin derived from the venom of the scorpion Buthus tamulus. Iberiotoxin binds to the extracellular vestibule of the BK channel, effectively blocking the flow of potassium ions through the channel.
Automatically generated - may contain errors
Lab products found in correlation
Flutamide, by Merck Group (1 mentions)
Theophylline, by Merck Group (1 mentions)
Caffeine, by Merck Group (1 mentions)
Sildenafil, by Merck Group (1 mentions)
Mdl 12 330a, by Merck Group (1 mentions)
Glibenclamide, by Santa Cruz Biotechnology (1 mentions)
Tadalafil, by Santa Cruz Biotechnology (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
3 protocols using iberiotoxin
1
Xanthine and Steroid Bioactive Compounds
2
Preparation of Pharmacological Agents
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KCl (Qualigens, Mumbai, India) was dissolved in double-distilled water to make a concentration of 168 mg/ml. KCl was prepared fresh before each experiment. Sildenafil (Sigma Aldrich, St Louis, MO, USA) was dissolved in dimethyl sulphoxide (DMSO), (Sigma Aldrich, St Louis, MO, USA) to give a concentration of 5 mg/ml. ODQ (Sigma Aldrich, St Louis, MO, USA) was dissolved in DMSO to give a concentration of 5 mg/ml. MDL-12,330A (Sigma, St Louis, MO, USA) in pure powder form was dissolved in DMSO to give a working concentration of 5 mg/ml. TEA (Qualigens, Mumbai, India) was dissolved in double-distilled water to give a concentration of 50 mg/ml. Iberiotoxin (Santa Cruz Biotechnology, Dallas, TX, USA) was dissolved in double-distilled water to give a 4 μM stock solution.
3
Preparation of Stock Solutions
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Potassium chloride (KCl; Qualigens, Mumbai, India), was dissolved in double-distilled water to obtain a concentration of 168 mg/ml. KCl was prepared fresh every day. Tadalafil (Santa Cruz Biotechnology, Dallas, TX, USA) was dissolved in DMSO (Sigma-Aldrich, St. Louis, MO, USA) to achieve a concentration of 5 mg/ml. Glibenclamide (Santa Cruz Biotechnology, Dallas, TX, USA) was dissolved in 90% ethanol to obtain a 2 mM stock solution. Iberiotoxin (Santa Cruz Biotechnology, Dallas, TX, USA) was dissolved in double-distilled water to obtain a 4 μM stock solution.
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