Superoxide dismutase sod

Commercial mouse enzyme-linked immunosorbent assay (ELISA) kits were used to measure albumin and the proinflammatory and/or fibrosis-related cytokines interleukin (IL)- 6, IL-1β, and tumor necrosis factor α (TNF-α; Thermo Fisher Scientific, Inc., Bartlesville, OK, USA) in BAL fluid, as well as myeloperoxidase (MPO; BioVision, Inc., Milpitas, CA, USA), the lipid peroxidation enzyme, malondialdehyde (MDA; Abcam, Burlingame, CA, USA), and the oxidative stress-related enzyme, superoxide dismutase (SOD; Bioassay Systems, Hayward, CA, USA) in lung tissues. The ELISAs were performed according to the manufacturers’ instructions.

AFB1 (≥99%) was supplied by Acros Organics [Gell, Belgium (Cat. No: 227340100)]. Curcumin (≥99%) was purchased by Sigma Aldrich [St. Louis, MO, USA (Cat. No: C1386)]. Malondialdehyde (MDA, Cat. No: E0156Ra), glutathione (GSH, Cat. No: E1101Ra), superoxide dismutase (SOD, Cat. No: E0168Ra) enzyme-linked immunosorbent assay (ELISA) kits were purchased from Bioassay Technology Laboratory (Shangai, China). Tumor necrosis factor-alpha (TNF-α, Cat. No: KRC3011), interleukin-1 beta (IL1β, Cat. No: BMS630), interleukin-6 (IL-6, Cat. No: BMS625) enzyme-linked immunosorbent assay (ELISA) kits were purchased from Thermo Fisher Scientific Inc. (USA). Alanine transaminase (ALT, Cat. No: DF143), aspartate transaminase (AST, Cat. No: DF41A), alkaline phosphatase (ALP, Cat. No: DC150), and gamma-glutamyl transferase (GGT, Cat. No: DF45A) kits were obtained from Siemens Medical System (Erlangen, Germany).