Antibodies for nf κb p65

UA (purity > 98%) was purchased from Chengdu Herbpurify Co. Ltd. (Chendu, China). Hoechst 33342, N-acetyl cysteine (NAC), LPS (Escherichia coli serotype 055:B5, LPS), 5-(6)-carboxy-2′, 7′-dichlorodihydrofluorescein diacetate (DCFH₂-DA), and BAY11-7082 were purchased from Sigma Aldrich (St. Louis, MO, USA). Antibodies for TLR4, MyD88 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies for NF-κB p65, GAPDH, and Histone H3 were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-LOX-1 antibody was obtained from R&D (Minneapolis, MN, USA). The BCA protein kits were purchased from Thermo Fisher (Suwanee, GA, USA). SiRNAs for TLR4, MyD88 were purchased from Gene Pharma Company (Shanghai, China). Oil Red O Staining Kit and HE Staining Kit were obtained from Nanjing Jiancheng Bioengineering Research Institute (Nanjing, China).

Antibodies for NF-κB p65 (#6956), p-NF-κB p65 (#3033) and β-Actin (#4970) (Cell Signaling Technology), p-TBK1 (#AF8190) and p-STAT3 (#AF3293) (Affinity Biosciences), STAT3 (#GB12176, Servicebio), HK2 (#22029-1-AP) and IRF3 (#11312-1-AP) (Proteintech Group), p-IRF3 (#MA5-14947, Invitrogen), TBK1 (#ab227182), Goat Anti-Rabbit IgG H&L (HRP) (#ab6721) and Rabbit Anti-Mouse IgG H&L(HRP) (#ab6728) (Abcam) were used for blotting. The anti-PDCoV-N antibodies were prepared in our laboratory. The Alexa Fluor 488-conjugated goat anti-mouse (#4408S) was purchased from Cell Signaling Technology. The STAT3 inhibitor static (#HY-13818) was purchased from Med Chem Express. The selenomethionine (SeMet) (#S3132) was purchased from Sigma. The miR-9-5p mimic, miR-125b-5p-1 mimic, and miR-125b-5p-1 inhibitor were purchased from Gene Pharma.