Anti-c-myc monoclonal antibody (Clontech, USA; 1:1,000), anti-GAPDH antibody (Cell Signaling Technology, USA; 1:10,000), anti-6X-His monoclonal antibody (Sigma; 1:2,000), anti-p24 monoclonal antibody (NIH AIDS Reagent Program, USA; 1:3,000), anti-PARP(Cell Signaling Technology, USA; 1:1000), anti-Rabbit IgG conjugated to HRP (Jackson Immunoresearch, USA; 1:10,000) and anti-Mouse IgG conjugated to HRP (Jackson Immunoresearch; 1:10,000) were used for the western blotting experiments.
Anti c myc monoclonal antibody
Manufactured by Takara Bio
Sourced in United States
The Anti-c-Myc Monoclonal Antibody is a laboratory reagent designed for the detection and identification of the c-Myc protein, a transcription factor involved in various cellular processes. This antibody can be used for applications such as Western blotting, immunoprecipitation, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Igg sepharose 6 fast flow beads, by GE Healthcare (2 mentions)
Sybr green, by Thermo Fisher Scientific (2 mentions)
Nprotein a sepharose 4 fast flow beads, by GE Healthcare (2 mentions)
Lightcycler 480 2, by Roche (2 mentions)
Anti rabbit igg conjugated to hrp, by Jackson ImmunoResearch (1 mentions)
Anti mouse igg conjugated to hrp, by Jackson ImmunoResearch (1 mentions)
Anti 6xhis monoclonal antibody, by Merck Group (1 mentions)
Anti gapdh antibody, by Cell Signaling Technology (1 mentions)
Anti parp, by Cell Signaling Technology (1 mentions)
4 protocols using anti c myc monoclonal antibody
1
Western Blot Antibody Conditions
2
Single-Molecule Actin-Myc Binding Assay
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For the step size and dwell time determinations, Qdot 525 conjugated with goat F(ab’)2 antimouse immunoglobulin G (H + L) were mixed with anti-c-Myc monoclonal antibody (Clontech) as described previously (33 (link), 34 (link)). F-actin was labeled by Alexa Fluor 568 Phalloidin at a 1:1 ratio overnight and stored at 4 °C. For single-molecule experiments with Qdot-labeled HM19, 7.5 μM labeled and unlabeled actins were mixed at the ratio of 1:10, and the mixture was diluted to 1 μM just before the experiments.
3
ChIP-qPCR for Protein-DNA Interactions
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ChIP-qPCR was performed essentially as previously described (Graf et al., 2017 (link)). For protein A ChIP, IgG Sepharose 6 Fast Flow beads (GE Healthcare) were used. For Myc ChIP, nProtein A Sepharose 4 Fast Flow beads (GE Healthcare) were used; after preclearing, 9 µl anti-c-Myc Monoclonal antibody (Clontech/Takara) were added to each sample. qPCR was performed using a LightCycler 480 II (Roche) and SYBR-Green (Thermo Scientific) detection with an annealing temperature of 60°C (40 cycles). Primers are listed in Supplementary file 1C . Measured Cq values were corrected to input and graphs were created using R. All strains were propagated for ~50 generations before ChIP-qPCR analysis.
4
ChIP-qPCR Protocol for Protein A and Myc
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ChIP-qPCR was performed essentially as previously described (Graf et al., 2017) (link). For protein A ChIP, IgG Sepharose 6 Fast Flow beads (GE Healthcare) were used. For Myc ChIP, nProtein A Sepharose 4 Fast Flow beads (GE Healthcare) were used; after preclearing, 9 µl anti-c-Myc Monoclonal antibody (Clontech/Takara) were added to each sample. qPCR was performed using a LightCycler 480 II (Roche) and SYBR-Green (Thermo Scientific) detection with an annealing temperature of 60ºC (40 cycles). Primers are listed in Supplementary Table S3. Measured Cq values were corrected to input and graphs were created using R. All strains were propagated for ~50 generations before ChIP-qPCR analysis.
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