Planar titanium nitride MEAs with 60 channels (59 recording electrodes plus one reference electrode; 200 μm inter-electrode distance, 30 μm electrode diameter) were obtained from Multichannel Systems (Reutlingen, Germany). For the developmental recordings, a standard 8×8 layout was used. For all other MEA recordings, a custom layout with two sub-arrays for cortex (8×4, 31 electrodes) and striatum (6×5, 28 electrodes) was used. Both sub-arrays were separated by 1,200 μm (Fig 3A ). Data were recorded at 25 kHz for MUA or 1 kHz for LFP using an MEA1060 amplifier and the MC Rack software (Multichannel Systems). Spontaneous activity for the developmental data (20 min) and the experiments with PTX bath application (4 μM, 60 min) was recorded in culture medium under sterile conditions. Washout recordings were done 24–48 h after the culture medium was replaced with conditioned medium collected 3–4 d before the experiment. All recordings were performed at 35 ± 0.5°C after 2 wk in vitro if not stated otherwise.
Mea1060 amplifier
Manufactured by MultiSciences Biotech
Sourced in Germany
The MEA1060 amplifier is a compact and versatile device designed for use in electrophysiology experiments. It features 60 channels for recording and stimulating neural signals, and is capable of amplifying and filtering these signals. The MEA1060 is a key component in multi-electrode array (MEA) systems, enabling researchers to study the electrical activity of neural networks.
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2 protocols using mea1060 amplifier
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Planar Titanium Nitride MEA Recordings
2
Electrical Activity of Cardiomyocyte Aggregates
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Multielectrode arrays (MEAs, Multi Channel Systems, Reutlingen, Germany) were plasma-treated and coated with gelatine (0.1% in D-PBS) for at least two hours at 37°C. The MEAs used in this study had 60 TiN electrodes with diameters of 30 µm and interelectrode distances of 200 µm arranged in 8 rows and 8 columns. Beating EBs were selected on day 12 of differentiation, incubated with IMDM supplemented with 20% FCS and allowed to attach to the MEA. After two days, electrical activity was measured using a MEA1060 amplifier (Multi Channel Systems, Reutlingen, Germany) for 10 min. Temperature was kept at 37°C. Data were digitised with a sampling frequency of 5 kHz and recorded with the MCRack software (Multi Channel Systems, Reutlingen, Germany). Interspike intervals between recorded field potentials were analysed off-line using custom-written routines in Matlab 5.3 (The Mathworks, Natick, MA, USA).
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