Quadromacs magnetic cellseparator

Manufactured by Miltenyi Biotec

The QuadroMACS magnetic cell separator is a laboratory instrument designed for the separation and enrichment of specific cell populations from complex biological samples. It utilizes magnetic beads coated with antibodies that bind to target cells, allowing their separation and isolation using a magnetic field. The QuadroMACS system enables efficient and reproducible cell separation, facilitating various research and diagnostic applications.

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Lab products found in correlation

Nucleocounter nc 200, by ChemoMetec (3 mentions) Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (3 mentions) Cd45 microbeads, by Miltenyi Biotec (3 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (2 mentions) Via1 cassette, by ChemoMetec (1 mentions)

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QuadroMACS (24 citations)

3 protocols using quadromacs magnetic cellseparator

CD45+ Cell Isolation and Enumeration

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Cells from donor 411c were pelleted for 5 minutes at
300g and resuspended in 80 µl of ice-cold MACS
buffer (0.5% BSA (Sigma-Aldrich Co. Ltd), 2 mM EDTA (ThermoFisher) in DPBS
(Gibco)) and 20 µl of CD45 Microbeads (Miltenyi Biotech). Cells were
incubated for 15 minutes at 4°C before being washed with 2 ml of MACS
buffer and centrifuged as above. Cells were resuspended in 500 µl of MACS
buffer and passed through a pre-wetted MS column on QuadroMACS magnetic cell
separator (Miltenyi). The column was washed 4 times with 500 µl of MACS
buffer, allowing the full volume of each wash to pass through the column before
the next wash. The column was removed from the magnet and the cells eluted with
force with 1 ml of MACS buffer into a 15 ml tube. Cells were pelleted as above
and cell number and viability were determined using a NucleoCounter NC-200 and
Via1-Cassette (ChemoMetec). Cells were resuspended at 500 cells/µl in
0.04% BSA in PBS.

James K.R., Gomes T., Elmentaite R., Kumar N., Gulliver E.L., King H.W., Stares M.D., Bareham B.R., Ferdinand J.R., Petrova V.N., Polański K., Forster S.C., Jarvis L.B., Suchanek O., Howlett S., James L.K., Jones J.L., Meyer K.B., Clatworthy M.R., Saeb-Parsy K., Lawley T.D, & Teichmann S.A. (2020). Distinct microbial and immune niches of the human colon. Nature immunology, 21(3), 343-353.

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CD45+ Cell Isolation and Enumeration

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Magnetic Separation of CD45+ Immune Cells

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Samples with a cell yield of greater than 500,000 were enriched by MACS. Dissociated cells were centrifuged for 5 min at 300g at 4 °C and resuspended in 80 μl chilled MACS buffer (D-PBS, 0.5% (w/v) BSA (Sigma-Aldrich, A7906-10G), 2 mM EDTA (Thermo Fisher, 15575020)) with 20 μl CD45 MicroBeads (Miltenyi Biotech, 130-045-801) and incubated for 15 min at 4 °C. Cells were washed with 2 ml MACS buffer and centrifuged as above and resuspended in 500 μl MACS buffer. Cells were passed through a pre-wetted MS column (Miltenyi Biotech, 130-042-201) on a QuadroMACS Magnetic Cell Separator (Miltenyi Biotech) followed by four rounds of 500 μl of MACS buffer. Flow-through was collected as the CD45⁻ fraction. The column was removed from the magnet and the CD45⁺ fraction was eluted with 1 ml of MACS buffer. CD45⁻ and CD45⁺ fractions were centrifuged as above and resuspended in 0.5–1 ml of 0.04% (w/v) BSA in D-PBS. Cell count and viability was determined using a NucleoCounter NC-200 and Via1-Cassette (ChemoMetec) or haemocytometer and resuspended in 0.04% (w/v) BSA in D-PBS. Fetal CD45⁺ and CD45⁻ fractions were combined at a 1:1 ratio.

Elmentaite R., Kumasaka N., Roberts K., Fleming A., Dann E., King H.W., Kleshchevnikov V., Dabrowska M., Pritchard S., Bolt L., Vieira S.F., Mamanova L., Huang N., Perrone F., Goh Kai’En I., Lisgo S.N., Katan M., Leonard S., Oliver T.R., Hook C.E., Nayak K., Campos L.S., Domínguez Conde C., Stephenson E., Engelbert J., Botting R.A., Polanski K., van Dongen S., Patel M., Morgan M.D., Marioni J.C., Bayraktar O.A., Meyer K.B., He X., Barker R.A., Uhlig H.H., Mahbubani K.T., Saeb-Parsy K., Zilbauer M., Clatworthy M.R., Haniffa M., James K.R, & Teichmann S.A. (2021). Cells of the human intestinal tract mapped across space and time. Nature, 597(7875), 250-255.

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