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Anti mouse cd80 alexa fluor 647

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Anti-mouse CD80-Alexa Fluor 647 is a fluorescently labeled antibody that binds to the mouse CD80 protein. CD80 is a costimulatory molecule expressed on antigen-presenting cells and is involved in T cell activation. The Alexa Fluor 647 dye can be used for flow cytometry and other fluorescence-based applications.

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Lab products found in correlation

Anti mouse cd86 percp, by BD (2 mentions) Anti mouse 1 a 1 e mhc 2 pe, by BD (2 mentions) Anti mouse cd40 fitc, by BD (2 mentions)

Close spelling variants of this product

Alexa Fluor 647 (58 citations) Anti-CD80 (43 citations) Alexa 647 (7 citations) Mouse anti (2 citations)

2 protocols using anti mouse cd80 alexa fluor 647

1

Immune Cell Activation Analysis

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RAW 264.7 macrophages and DC2.4 DCs were respectively seeded in 12-well plates and incubated overnight for experiment. Svg3 was transfected using Lipofectamine reagent at a final concentration of 25 nM. After treatment for 24 h, the cells were collected and stained with anti-mouse CD86-PerCp, anti-mouse CD80-Alexa Fluor 647, anti-mouse CD40-FITC, or anti-mouse I-A/I-E (MHC II)-PE, respectively, for flow cytometry (BD Canto).
Zhou S., Su T., Cheng F., Cole J., Liu X., Zhang B., Alam S., Liu J, & Zhu G. (2024). Engineering cGAS-agonistic oligonucleotides as therapeutics for cancer immunotherapy. Molecular Therapy. Nucleic Acids, 35(1), 102126.
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2

Macrophage and DC Activation Assay

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RAW 264.7 macrophages and DC2.4 DCs were respectively seeded in 12-well plate and incubated overnight for experiment. Svg3 was transfected using lipofectamine reagent at a final concentration of 25 nM. After treatment for 24 h, the cells were collected and stained with anti-mouse CD86-PerCp, anti-mouse CD80-Alexa Fluor 647, anti-mouse CD40-FITC, anti-mouse I-A/I-E (MHC II)-PE respectively for flow cytometry (BD Canto).
Zhou S., Su T., Cheng F., Cole J., Liu X., Zhang B., Alam S., Liu J, & Zhu G. (2023). Engineering cGAS-agonistic oligonucleotides as therapeutics and vaccine adjuvants for cancer immunotherapy. bioRxiv.
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