Cells were lysed in NT2 buffer containing 50 mmol/L Tris, pH 7.4, 150 mmol/L NaCl, 1 mmol/L MgCl2, 0.05% Nonidet P-40, 1 mmol/L sodium orthovanadate, 0.5 mmol/L dithiothreitol, 1 mmol/L phenylmethylsulfonyl fluoride, 2 μg/mL aprotinin, 2 μg/mL leupeptin, and 2 μg/mL pepstatin. Proteins from cell lysates were separated by 10% SDS-PAGE gel electrophoresis (Bio-Rad), and transferred to nitrocellulose membranes (Invitrogen). Immunoblots were probed with antibodies recognizing: Aiolos (Cell Signaling Technology), Ikaros (Millipore), IRF7 (Cell Signaling Technology), DDX58 (Thermo Scientific), IFIT3 (Novus Biologicals), c-myc (Abcam), IRF4 (Santa Cruz), p65 (Cell Signaling Technology), p-p65 Ser236 (Cell Signaling Technology), p105/p50 (Cell Signaling Technology), p100/p52 (Cell Signaling Technology), Tubulin (Cell Signaling Technology), TBP (Cell Signaling Technology), and β-actin (Li-Cor). Signals were detected with a Li-Cor Odyssey imager or WES (Protein Simple).
Ikaros
Manufactured by Merck Group
Sourced in United States
Ikaros is a laboratory equipment product developed by Merck Group. It is designed for use in various scientific and research applications. Ikaros is a versatile and reliable piece of equipment that serves as a core function in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by LI COR (2 mentions)
C myc, by Abcam (2 mentions)
Ddx58, by Thermo Fisher Scientific (1 mentions)
Nitrocellulose membrane, by Thermo Fisher Scientific (1 mentions)
Sds page gel electrophoresis, by Bio-Rad (1 mentions)
Ifit3, by Novus Biologicals (1 mentions)
Aiolos, by Cell Signaling Technology (1 mentions)
Aiolos, by Santa Cruz Biotechnology (1 mentions)
2 protocols using ikaros
1
Immunoblot Analysis of Cellular Signaling
2
Immunoblotting Antibody Variability Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Several antibodies were used for immunoblotting in these experiments including *Ikaros (Millipore, Billerica, MA, USA; Abcam, Cambridge, MA, USA; Santa Cruz Biotechnology, Dallas, TX, USA), Aiolos (Santa Cruz Biotechnology and Celgene developed), IRF4 (Santa Cruz Biotechnology), c-Myc (Abcam) and β-actin (LI-COR Biotechnology, Lincoln, NE, USA). It is noteworthy that several antibodies for both Ikaros and Aiolos exhibited varying degrees of background bands that varied from source and lot-to-lot. In particular, variability was greater for nearly all immunoblots of Ikaros, where there are mostly two bands (only one band for some cell lines) at the predicted molecular weight (likely isoforms) and where sometimes both bands are degraded with drug treatment and others only show degradation of one band.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!