We monitored nine distinct locations along a 11.62 km reach of the River Cam, featuring sites upstream, downstream and within the urban belt of the city of Cambridge, UK. Measurements were taken at three time points, in two-month intervals between April and August 2018 (Figure 1 ; Supplementary file 1a ). To warrant river base flow conditions and minimise rain-derived biases, a minimum dry weather time span of 48 hr was maintained prior to sampling (Fisher et al., 2015 (link)). One litre of surface water was collected in autoclaved DURAN bottles (Thermo Fisher Scientific, Waltham, MA, USA), and cooled to 4°C within 3 hr. Two bottles of water were collected consecutively for each time point, serving as biological replicates of location 9 (samples 9.1 and 9.2).
Duran bottles
Manufactured by Thermo Fisher Scientific
Sourced in United States
DURAN bottles are laboratory glassware products designed for general storage, mixing, and handling of liquids and powders in scientific research and testing applications. They are made of borosilicate glass, which provides chemical resistance and durability. DURAN bottles are available in a range of sizes and formats to accommodate different volume requirements.
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Lab products found in correlation
Agilent 1200 series controller, by Agilent Technologies (1 mentions)
Sevencompact ph ion s220, by Thermo Fisher Scientific (1 mentions)
Hereus biofuge primo r, by Thermo Fisher Scientific (1 mentions)
Agilent 1200 series autosampler, by Agilent Technologies (1 mentions)
Agilent 1200 series dad detector, by Agilent Technologies (1 mentions)
Agilent 1200 series binary pump, by Agilent Technologies (1 mentions)
Chemstation software, by Agilent Technologies (1 mentions)
2 protocols using duran bottles
1
River Cam Water Sampling Protocol
2
Partition Experiments for Dissolution Analysis
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Partition experiments were run in triplicate at 37 °C, using 50 mL Corning ® PP selfstanding centrifuge tubes, the USP 2 paddle apparatus (Agilent 708-DS Dissolution Apparatus) or 100 mL glass DURAN ™ bottles (Fischer). All samples were analysed in an HPLC system consisting of an Agilent 1200 series binary pump (G1312A), an Agilent 1200 series DAD detector (G1315D), an Agilent 1200 series autosampler (G1329A), an Agilent 1200 series controller (G1316A) and Chemstation software (Agilent Technologies, Santa Clara, United States). A pH meter Mettler-Toledo AG (model SevenCompact pH/Ion S220, Schwerzenbach, Switzerland), a centrifuge Hereus Biofuge Primo R (Thermo Scientific, Hanau, Germany) and a vortex mixer Rotamixer (HTZ, Cheshire, UK) were used.
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