Three small interfering RNAs (siRNAs) targeting circ_0007841, including si-circ_0007841#1 (5′-UGUUAGUUGCAAUGAAGAGAG-3′), si-circ_0007841#2 (5′-UAAUGAUCAUGCCAAAUACUC-3′) and si-circ_0007841#3 (5′-UCACAUACACGAUAGACUGGC-3′), its negative control (si-NC), circ_0007841 overexpression plasmid (circ_0007841), its control (Vector), BRD4 overexpression plasmid (BRD4), its control (pcDNA), miR-338-3p mimics (miR-338-3p), its control miR-NC, miR-338-3p inhibitor (in-miR-338-3p) and its control in-miR-NC were obtained from Genepharma (Shanghai, China). MM cells were seeded into 24-well plates at a density of 2 × 105 cells/well overnight, and transfection was conducted with Lipofectamine 3000 (Invitrogen).
Mir 338 3p inhibitor in mir 338 3p
Manufactured by GenePharma
Sourced in China
The MiR-338-3p inhibitor (in-miR-338-3p) is a laboratory reagent designed to inhibit the expression of the microRNA molecule miR-338-3p. This inhibitor can be used to study the biological functions and regulatory roles of miR-338-3p in various experimental settings.
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Lab products found in correlation
3 protocols using mir 338 3p inhibitor in mir 338 3p
1
Silencing circ_0007841 in Multiple Myeloma Cells
2
Targeting Circular RNA in Multiple Myeloma
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Three small interfering RNAs (siRNAs) targeting circ_0007841, including si-circ_0007841#1 (5'-UGUUAGUUGCAAUGAAGAGAG-3'), si-circ_0007841#2 (5'-UAAUGAUCAUGCCAAAUACUC-3') and si-circ_0007841#3 (5'-UCACAUACACGAUAGACUGGC-3'), its negative control (si-NC), circ_0007841 overexpression plasmid (circ_0007841), its control (Vector), BRD4 overexpression plasmid (BRD4), its control (pcDNA), miR-338-3p mimics (miR-338-3p), its control miR-NC, miR-338-3p inhibitor (in-miR-338-3p) and its control in-miR-NC were obtained from Genepharma (Shanghai, China). MM cells were seeded into 24-well plates at a density of 2 × 10 5 cells/well overnight, and transfection was conducted with Lipofectamine 3000 (Invitrogen).
Cell counting kit-8 (CCK8) assay MM cells were plated in 96-well plates at the density of 5 × 10 3 cells/well and cultured overnight. After transfection for indicated time points (0 h, 24 h, 48 h or 72 h), MM cells were incubated with 10 μL CCK-8 (Sigma, St. Louis, MO, USA) for 4 h. The absorbance at 450 nm was detected by a microplate reader (BioTek, Winooski, VT, USA).
Cell counting kit-8 (CCK8) assay MM cells were plated in 96-well plates at the density of 5 × 10 3 cells/well and cultured overnight. After transfection for indicated time points (0 h, 24 h, 48 h or 72 h), MM cells were incubated with 10 μL CCK-8 (Sigma, St. Louis, MO, USA) for 4 h. The absorbance at 450 nm was detected by a microplate reader (BioTek, Winooski, VT, USA).
3
Targeting Circular RNA in Multiple Myeloma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three small interfering RNAs (siRNAs) targeting circ_0007841, including si-circ_0007841#1 (5'-UGUUAGUUGCAAUGAAGAGAG-3'), si-circ_0007841#2 (5'-UAAUGAUCAUGCCAAAUACUC-3') and si-circ_0007841#3 (5'-UCACAUACACGAUAGACUGGC-3'), its negative control (si-NC), circ_0007841 overexpression plasmid (circ_0007841), its control (Vector), BRD4 overexpression plasmid (BRD4), its control (pcDNA), miR-338-3p mimics (miR-338-3p), its control miR-NC, miR-338-3p inhibitor (in-miR-338-3p) and its control in-miR-NC were obtained from Genepharma (Shanghai, China). MM cells were seeded into 24-well plates at a density of 2 × 10 5 cells/well overnight, and transfection was conducted with Lipofectamine 3000 (Invitrogen).
Cell counting kit-8 (CCK8) assay MM cells were plated in 96-well plates at the density of 5 × 10 3 cells/well and cultured overnight. After transfection for indicated time points (0 h, 24 h, 48 h or 72 h), MM cells were incubated with 10 μL CCK-8 (Sigma, St. Louis, MO, USA) for 4 h. The absorbance at 450 nm was detected by a microplate reader (BioTek, Winooski, VT, USA).
Cell counting kit-8 (CCK8) assay MM cells were plated in 96-well plates at the density of 5 × 10 3 cells/well and cultured overnight. After transfection for indicated time points (0 h, 24 h, 48 h or 72 h), MM cells were incubated with 10 μL CCK-8 (Sigma, St. Louis, MO, USA) for 4 h. The absorbance at 450 nm was detected by a microplate reader (BioTek, Winooski, VT, USA).
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