Log In Sign up
The largest database of trusted experimental protocols

Phenylephrine hydrochloride

Manufactured by Bio-Techne
Visit Supplier
Sourced in Germany

Phenylephrine hydrochloride is a chemical compound used in various laboratory applications. It serves as a vasoconstrictor, which means it can constrict blood vessels. This property makes it a useful tool for researchers working in fields such as cardiovascular biology and neuroscience.

Automatically generated - may contain errors

Lab products found in correlation

Dexmedetomidine hydrochloride, by Bio-Techne (3 mentions) Prazosin hydrochloride, by Bio-Techne (2 mentions) Tetrodotoxin citrate, by Alomone (2 mentions) Chlorprothixene hydrochloride, by Merck Group (2 mentions) Nbqx disodium, by Bio-Techne (2 mentions) Sr 95531 hydrobromide, by Bio-Techne (2 mentions) Rs cpp, by Bio-Techne (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Nadolol, by Merck Group (1 mentions) Doxazosin mesylate, by Bio-Techne (1 mentions) Isoproterenol, by Merck Group (1 mentions) L748337, by Bio-Techne (1 mentions) Brl37344, by Merck Group (1 mentions) Rs79948 hydrochloride, by Bio-Techne (1 mentions) Norepinephrine, by Merck Group (1 mentions)

3 protocols using phenylephrine hydrochloride

1

In vivo and in vitro imaging of neuronal activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
For in vivo i.p. injection, chlorprothixene hydrochloride (Sigma-Aldrich, #C1671), prazosin hydrochloride (Tocris, #0623) and dexmedetomidine hydrochloride (Tocris, 2749) were dissolved in DMSO (Sigma-Aldrich, #D2650) at 5 mg/mL, 3 mg/mL and 0.1 mg/mL, respectively. First, 5 minutes of baseline activity was measured using 2P microscopy and then the animals were i.p. injected with equal amounts of DMSO per body weight (1 μL per 1 μg b.w.). Animals were returned to their home cage after injection and then re-mounted under the 2P microscope 20 min after injection for 5 minutes of imaging. For in vitro cortical slice imaging, phenylephrine hydrochloride (Tocris, #2838), tetrodotoxin citrate (Alomone labs #T-550), NBQX disodium (Tocris, #1044), (RS)-CPP (Tocris, #0173) and SR 95531 hydrobromide (Tocris, 1262) were dissolved in ACSF and applied through the superfusing solution.
Lu T.Y., Hanumaihgari P., Hsu E.T., Agarwal A., Kawaguchi R., Calabresi P.A, & Bergles D.E. (2023). Norepinephrine modulates calcium dynamics in cortical oligodendrocyte precursor cells promoting proliferation during arousal in mice. Nature neuroscience, 26(10), 1739-1750.
+ Open protocol
+ Expand
2

In vivo and in vitro Imaging Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
For in vivo i.p. injection, chlorprothixene hydrochloride (Sigma-Aldrich, #C1671), prazosin hydrochloride (Tocris, #0623) and dexmedetomidine hydrochloride (Tocris, 2749) were dissolved in DMSO (Sigma-Aldrich, #D2650) at 5 mg/mL, 3 mg/mL and 0.1 mg/mL, respectively. First, 5 minutes of baseline activity was measured using 2P microscopy and then the animals were i.p. injected with equal amounts of DMSO per body weight (1 μL per 1 μg b.w.). Animals were returned to their home cage after injection and then re-mounted under the 2P microscope 20 min after injection for 5 minutes of imaging. For in vitro cortical slice imaging, phenylephrine hydrochloride (Tocris, #2838), tetrodotoxin citrate (Alomone labs #T-550), NBQX disodium (Tocris, #1044), (RS)-CPP (Tocris, #0173) and SR 95531 hydrobromide (Tocris, 1262) were dissolved in ACSF and applied through the superfusing solution.
Lu T.Y., Hanumaihgari P., Hsu E.T., Agarwal A., Kawaguchi R., Calabresi P.A, & Bergles D.E. (2023). Norepinephrine modulates calcium dynamics in cortical oligodendrocyte precursor cells promoting proliferation during arousal in mice. Nature neuroscience, 26(10).
+ Open protocol
+ Expand
3

Adrenoceptor Pharmacology Toolbox

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phenylephrine hydrochloride (selective α 1 AR agonist), dexmedetomidine hydrochloride (selective α 2 AR agonist), L-748, 337 (β 3 AR antagonist), doxazosin mesylate (α 1 /α 2 AR antagonist) and RS79948 hydrochloride (selective α 2 AR antagonist) were obtained from Tocris/Bio-Techne (Wiesbaden, Germany).
Isoproterenol (unselective β-AR agonist), norepinephrine (unselective AR agonist), BRL 37344 (selective β 3 -AR agonist) and nadolol (non-selective β AR agonist) were obtained from Sigma Aldrich (St. Louis, USA).
Ding L., Lowin T., Cheng X., Claßen T., & Pongratz G. (2021). Adrenergic receptors in osteoarthritic and rheumatoid synovial fibroblasts: Identification of β3 as novel player to modulate IL-6 and p38 activation.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!