Polylactic acid (molecular weight: 300,000, Cat No. 1604000258) was purchased from Corbion (Netherlands). Gelatin (sourced from pigskin, type A, ~228 g bloom, viscosity (6.67%, 60°C): 3.8 mPas, Cat No. 1010583) was purchased from GELITA (United States, US). Hexafluoroisopropanol (HFIP, Analytical Pure) was purchased from Halocarbon Company (USA). Poly (lactic acid) and gelatin at a mass ratio of 7:3 were added to HFIP, stirred at room temperature for 12 h until completely dissolved, and the spinning solution was prepared to have a total concentration of 8% (w/v). Dulbecco's modified eagle medium (DMEM) high-glucose medium, fetal bovine serum, pancreatin, and phosphate buffer were purchased from Gibco (USA); Alamar Blue was purchased from Maibio (Shanghai, China); the CCK-8 kit was purchased from Dojindo (Japan). The live/dead cell viability staining kit was purchased from KeyGen Biotech (Nanjing, China).
Polylactic acid
Manufactured by Corbion
Sourced in Netherlands
Polylactic acid is a biodegradable and renewable polymer derived from lactic acid. It can be used as a raw material for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Phosphate buffer, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium dmem high glucose medium, by Thermo Fisher Scientific (1 mentions)
Pancreatin, by Thermo Fisher Scientific (1 mentions)
Cck 8 kit, by Dojindo Laboratories (1 mentions)
Cellmask deep red, by Thermo Fisher Scientific (1 mentions)
2 protocols using polylactic acid
1
Fabrication of PLA/Gelatin Blended Scaffolds
2
Mechanically Stimulated Cell Culture
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The fabricated scaffolds were cut into rectangle pieces (10 x 35 mm 2 to fit into the wells of 6-well culture plates suitable for mechanical stress culture using the Flexcell Tension system (BioFlex culture plate, cat. No. BF-3001C). The scaffolds were glued to the well-plate membrane using 6 wt% polylactic acid (Corbion, Netherlands) in chloroform. After drying of the glue and evaporation of chloroform, all samples were sterilized by soaking in 70% ethanol and incubated on a shaking platform for 10 min. Then, the scaffolds were washed twice with PBS. Before cell incubation with samples, the prepared scaffolds were next soaked in the cell culture medium overnight on the shaking platform to ensure the removal of residual ethanol. After that, 8 × 10 5 cells were seeded on the scaffolds glued in plate wells and were cultured overnight. After that, the scaffolds were subjected to an intermittent deformation of 5% at a frequency of 0.5 Hz for 48 h.
The cells were fixed with 4% paraformaldehyde (Biolegend fixation buffer, cat. No. 420801), permeabilized in Triton X-100, and stained with DAPI and Cellmask deep red (Invitrogen, cat. No.
The cells were fixed with 4% paraformaldehyde (Biolegend fixation buffer, cat. No. 420801), permeabilized in Triton X-100, and stained with DAPI and Cellmask deep red (Invitrogen, cat. No.
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