24sure microarray pack

Embryos presenting ≥5 cells and <30% fragmentation at 66 ± 2 h post-ICSI were considered suitable for biopsy. Embryos with <5 cells and/or >30% fragmentation were discarded. Zona opening was performed by laser thermolysis (Boada et al., 1998) and the biopsy was carried out by aspiration of a single blastomere. Whole genome amplification, processing and analysis were performed using the kits, software, and protocols for array comparative genomic hybridization (aCGH) analysis provided by the manufacturer (SurePlex® DNA Amplification System, 24Sure® Microarray Pack, Bluefuse®, Illumina®). One or two euploid embryos were replaced under ultrasound control (Coroleu et al., 2006) on day 5. Remaining euploid blastocysts were vitrified using the kits and protocols provided by the manufacturer (Kitazato®). Luteal phase support was Transition from blastomere to trophectoderm biopsy 193 achieved with vaginal progesterone (200 mg every 8 h) until the determination of beta-hCG in plasma (15 days after oocyte retrieval). For positive results, the support treatment continued until ultrasound detection of fetal heartbeat on the sixth week of pregnancy.