ROS in colon tissues was identified using dihydroethidium (DHE) (Nanjing Jiancheng). A 0.5-cm colon segment was subjected to fixation with 10% formalin for 24 h. The colon segment was then embedded in paraffin, sliced into 5-μm slices, incubated with DHE dye (Servicebio, China) for 30 min at 37°C, and then stained with DAPI for 10 min. A fluorescence microscope was used to view the slides.
Dhe dye
Manufactured by Wuhan Servicebio Technology
Sourced in China
DHE dye is a fluorescent dye commonly used for nucleic acid staining in various laboratory applications. It binds to DNA and emits a red fluorescence when excited by an appropriate light source.
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Lab products found in correlation
3 protocols using dhe dye
1
Visualizing Colon ROS Using DHE
2
Measuring Reactive Oxygen Species in Tumor Tissue
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According to Servicebio's established procedures, dihydroethidium (DHE) was used to identify reactive oxygen species (ROS) in tumor tissue. Fresh frozen colon tissues were embedded in an optimum cutting temperature compound (OCT, Sakura) and sectioned at a thickness of 5 μm thick. After washing, the sections were incubated with DHE dye (Servicebio) for 30 min at 37°C, followed by DAPI for 10 min. A fluorescent microscope was used to view the slides. Image Pro Plus was used to determine the mean fluorescence intensity (MFI) of DHE in three randomly chosen locations for each group.
3
Detecting ROS in Tumor Tissue by DHE Staining
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The ROS in tumor tissue was detected by dihydroethidium (DHE) according to the standard protocols by Servicebio. Fresh frozen tumor tissues were embedded in optimal cutting temperature compound (OCT, Sakura) and cut into 5 μm-thick sections. The sections were incubated with DHE dye (Servicebio) at 37°C for 30 min, followed by incubating with DAPI for 10 min after washing. The sections were mounted and sealed with an anti-fluorescence quenching agent. The slides were visualized by a fluorescence microscope. The mean fluorescence intensity (MFI) of DHE in 3 randomly selected areas for each group was calculated by Image Pro Plus.
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