Sections were examined using a Zeiss LSM510 Axioplan confocal laser scanning microscope. Cells containing multiple parasites were imaged in three dimensions (z-stacking) to allow precise counting of amastigotes (using the 63x or 100x objectives with appropriate scan zoom for the particular cell/number of parasites). Phase images were obtained at lower magnification (40x) to allow orientation of the tissue section and identification of specific layers/structures. All images were acquired using Zeiss LSM510 software. Scale bars were added using the Zeiss LSM Image Browser overlay function and the images were then exported as .TIF files to generate the figures. Movies were created either from the z-stack itself or using the projection utility of the Zeiss LSM Image Browser with z-stack source files.
Lsm510 axioplan confocal laser scanning microscope
Manufactured by Zeiss
The LSM510 Axioplan is a confocal laser scanning microscope designed for high-resolution imaging. It features a laser-based optical system that allows for the capture of detailed, three-dimensional images of samples. The LSM510 Axioplan is capable of producing high-quality, low-noise images with precise control over depth of field and resolution.
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Lab products found in correlation
2 protocols using lsm510 axioplan confocal laser scanning microscope
1
Confocal Microscopy for Parasite Quantification
2
Confocal Imaging of Intracellular Parasites
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Slides and sections were examined using a Zeiss LSM510 Axioplan confocal laser scanning microscope. Cells containing multiple parasites were imaged in three dimensions to allow precise counting of amastigotes (using the 63x or 100x objectives with appropriate scan zoom for the particular cell/number of parasites). Phase images were obtained at lower magnification (40x) to allow orientation of the tissue section and identification of specific layers/structures. All images were acquired using Zeiss LSM510 software. Scale bars were added using the Zeiss LSM Image Browser overlay function and the images were then exported as .TIF files to generate the Figs.
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