Annexin 5 fitc pi apoptosis double staining kit

D-galactose (D-gal), lipopolysaccharide (LPS), sodium lauryl sulfate (SLS), 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA), copper sulfate pentahydrate (CuSO4·5H2O) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell-countingkit-8 (CCK-8) was obtained from Dojindo Laboratories (Japan). Quantitative real-time polymerase chain reaction (qRT-PCR) primers were purchased from Generay Biotech Co., Ltd (Shanghai, CHINA). GeneJET RNA purification kit was bought from Thermo Fisher Scientific (USA). RNA Reverse Transcription Kit was obtained from PrimeScript™ RT reagent Kit (Takara, Japan). ELISA kits were bought from NeoBioscience (Xinbosheng, China). NO assay kit, NOS kit, LDH assay kit, β-gal staining kit, and Annexin V-FITC/PI apoptosis double staining kit were purchased from Beyotime (Jiangsu, China). All chemicals were used as received without further purification.

Tetrabutyl titanate were obtained from Aladdin (Shanghai, China). (3‐Aminopropyl) trimethoxysilane (APTMS) was purchased from Macklin (Shanghai, China). Titanium butoxide (GC, 99.0%), hydrogen peroxide (H₂O₂), heptanoic acid (AR, 98.0%), ethanol, N‐(3‐dimethylaminopropyl)‐N‐ethylcarbodiimide hydrochloride (EDC), doxorubicin (DOX) and N‐hydroxysuccinimide (NHS) were purchased from Aladdin (Shanghai, China). Cantharidin (CTD) was purchased from Sigma (St. Louis, MO, USA). NHS‐PEG₂₀₀₀‐COOH was purchased from Ponsure Biotechnology Co., Ltd (Shanghai, China). The BCA Kit and 4′,6‐Diamidino‐2‐phenylindole (DAPI) were obtained from Solarbio (Beijing, China). Cell Counting Kit‐8 (CCK‐8) and DCFH‐DA were purchased from Beyotime Biotechnology Co., Ltd (Shanghai, China). Fetal bovine serum (FBS), RPMI 1640, DMEM, trypsin‐EDTA and penicillin‐streptomycin were obtained from Zhejiang Tianhang Biotechnology Co. Ltd (Zhejiang, China). The YSA peptide (YSAYPDSVPMMSK) was obtained from GL Biochem Peptide Co., Ltd (Shanghai, China). The calcein‐AM/PI double staining kit was obtained from Yeasen (Shanghai, China). Annexin V‐FITC/PI apoptosis double staining kit was obtained from Beyotime Institute of Biotechnology (Shanghai, China). The abbreviations are summarized in Table 1.