Mouse livers were stained with rabbit anti-CD11b Ab (Abcam, Cambridge, MA), rat anti-Ly6G Ab (BD Biosciences, San Jose, CA), rabbit anti-4-hydroxynonenal Ab (Abcam), rabbit anti-HO-1 Ab (Enzo Life Sciences, Farmingdale, NY) and rat anti-CD68 Ab (Bio Rad, Hercules, CA). Human livers were stained with rabbit anti-HO-1 Ab (Enzo Life Sciences) and mouse anti-CD68 Ab (BD Biosciences). Signals were visualized with secondary Alexa Fluor Abs. Liver-infiltrating CD11b+ and Ly6G+ cells were scored semi-quantitatively by blindly counting positive cells in 10 HPF/section (x400).
Kageyama S., Hirao H., Nakamura K., Ke B., Zhang M., Ito T., Aziz A., Oncel D., Kaldas F.M., Busuttil R.W., Sosa R.A., Reed E.F., Araujo J.A, & Kupiec-Weglinski J.W. (2018). Recipient HO-1 Inducibility is Essential for Post-Transplant Hepatic HO-1 Expression and Graft Protection: From Bench-to-Bedside. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 19(2), 356-367.
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