Txnip sirna

To knock down the expression of NLRP3 or thioredoxin-interacting protein (TXNIP), MCs were transfected with NLRP3-small interfering RNA (siRNA) or TXNIP-siRNA (RiboBio, Co., Ltd., Guangzhou, China) with Lipo3000 (Invitrogen, Carlsbad, CA, USA, L3000-015) according to the manufacturer’s instructions. MCs transfected with NC-siRNA (RiboBio. Co., Ltd., China) were taken to be the transfection control (siNC). The untreated MCs were set as normal control. The subsequent experiments were performed 24 h after transfection completion. The sequences of each siRNA are listed in Supplementary Table 1. We designed three small interfering RNA sequences targeting NLRP3 and TXNIP and selected the siRNA sequence with the highest interference efficiency for subsequent transfection (Supplementary Figure 1 and Figure 1).