HKCI-2 and HKCI-10 cells were stably infected with OGT-shRNA or control-shRNA for 48 h. The cell lysis solution (100 ll) was loaded into the cartridges (Waters Co., Milford, MA). The aqueous plug was pulled from the solid phase extraction (SPE) cartridges with high and low vacuum respectively in the cartridges. SPE cartridges were eluted using methanol and ethyl acetate, respectively, thereafter combined, evaporated, redissolved and centrifuged. The supernatant was transferred to auto-sampler vials and stored at À80°C until analysis. An Agilent 1290 Ultra-high Performance Liquid Chromatography (UHPLC, Agilent Technologies, CA) was employed for the separation of components of each sample. The mass spectrometry was conducted on a 6550 iFunnel Q-TOF LC/MS system (Agilent Technologies, Santa Clara, CA) with a dual Agilent Jet Stream electrospray ion source (dual AJS ESI). The mass spectra were recorded across the range of 100-1700 m/z for qualitative analyses in both of negative and positive mode.
Agilent 1290 ultra high performance liquid chromatography
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 1290 Ultra-high Performance Liquid Chromatography (UHPLC) system is a high-performance liquid chromatography instrument designed for rapid and efficient separation and analysis of complex samples. It features advanced components and technology to deliver superior resolution, sensitivity, and speed in liquid chromatography applications.
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3 protocols using agilent 1290 ultra high performance liquid chromatography
1
Profiling OGT Knockdown in HKCI Cell Lines
2
Comprehensive Analytical Characterization of AuNPs
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The SERS spectra were obtained by an RmTracer-200-HS portable Raman spectrometer combined with a 785 nm excitation wavelength diode-stabilized stimulator (Opto Trace Technologies, Inc., Mountain View, CA, USA). The optical absorption measurements were carried out by a TU-1901 Ultraviolet Spectrophotometer (Beijing General Instrument Co., Ltd., Beijing, China). Morphological features of the as-produced AuNPs structures were characterized with an FEI Tecnai G2 F20 S-TWIN transmission electron microscope (FEI Company, Hillsboro, OR, USA). DM and CBF residues in soil were detected using Agilent 1290 Ultra High Performance Liquid Chromatography combined with a Photodiode Array Detector (Agilent Technology Co., Ltd., Santa Clara, CA, USA).
3
Comprehensive Analytical Techniques for Biomolecules
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All samples that needed to be dried were treated with an electric drying oven (Humgine, China). Amino acids were analyzed using Agilent 1290 Ultra High-Performance Liquid Chromatography (UPLC) (Agilent, USA), equipped with an automatic liquid sampler, a fluorescence detector and a diode array detector. Trace elements were determined by NexION 300X (PerkinElmer, USA) Inductively coupled plasma mass spectrometry (ICP-MS), equipped with a cyclonic spray chamber and a Meinhard nebulizer. The MARS microwave digestion instrument (CEM, USA) was used for acid digestion of samples. The polysaccharides and total alkaloids were measured using an ultraviolet-visible spectrophotometer (Thermo Scientific GENESYS, USA).
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