Cg k peg3 tamra ggrgds adhesion peptide

Borosilicate glass was chosen as a model material because of its transparency, allowing its use for microscopy experiments. Borosilicate glass slides (76 x 26 mm, thickness ≈ 1 mm) were obtained from Schott (Tempe, AZ, USA). These slides were then laser-cut into 10x10 mm pieces to fit in 24 well cell culture plates. H2O2 (33 wt %), concentrated H2SO4, acetone, ethanol, anhydrous toluene, dimethylsulfoxyde (DMSO), 3-aminopropyltriethoxysilane (APTES) and succinimidyl-4-(pmaleimidophenyl) butyrate (SMPB) were all purchased from Sigma-Aldrich, France. The fluorophore-tagged CG-K(PEG3-TAMRA)-GGRGDS adhesion peptide (referred to as RGD-TAMRA; MW 1437 g/mol) was synthesized by Anaspec (Fremont, CA, USA). CKIPKASSVPTELSAISMLYL and fluorophore-tagged CKIPKASSVPTELSAISMLYLK-FITC peptides (referred to as BMP-2 mimetic peptide; MW 2251.75 g/mol and BMP-2-FITC mimetic peptide; MW 2769 g/mol, respectively) were produced by Genecust, Belgium. These peptides were first dissolved at 2 mM in DMSO and stored at -20 °C until use. Peptides, to be conjugated with material surface, were resuspended at 20 µM in a 0.2 µm-filtered Phosphate-Buffered Saline (PBS, Life Technologies, France) solution containing 7.5 % glycerol.

Borosilicate glass slides (76 x 26 mm, thickness ≈ 1 mm) were obtained from Schott (Tempe, AZ, USA). These slides were laser-cut into 10x10 mm pieces, to fit in 24 well cell culture plates, by ALPhANOV, France. H2O2 (33 wt%), concentrated H2SO4, acetone, ethanol, anhydrous toluene, dimethylsulfoxyde (DMSO), 3-aminopropyltriethoxysilane (APTES) and succinimidyl-4-(pmaleimidophenyl) butyrate (SMPB) were all purchased from Sigma-Aldrich, France. The fluorophore-tagged CG-K(PEG3-TAMRA)-GGRGDS adhesion peptide (referred to as RGD-TAMRA; m.w = 1437 g mol -1 ) was synthesized by Anaspec (Fremont, CA, USA). CKIPKASSVPTELSAISMLYL and fluorophore-tagged CKIPKASSVPTELSAISMLYLK-FITC peptides (referred to as BMP-2 mimetic peptide; m.w = 2251.75 g mol -1 and BMP-2-FITC mimetic peptide; m.w = 2769 g mol -1 , respectively) were produced by Genecust, Luxemburg. These peptides were first dissolved at (2 mM) in DMSO and stored at -20 °C until use. Peptides, to be conjugated with material surface, were resuspended at (20 µM) in a 0.2 µm-filtered Phosphate-Buffered Saline (PBS, Life technologies, France) solution containing 7.5% glycerol.