Peg2000 cer16

Manufactured by Avanti Polar Lipids

Sourced in Germany

PEG2000-Cer16 is a polyethylene glycol-conjugated ceramide compound. It is a lipid-based material designed for use in various research and development applications.

Automatically generated - may contain errors

Lab products found in correlation

Hepes, by Merck Group (4 mentions) Sodium citrate, by Merck Group (4 mentions) Sodium phosphate, by Merck Group (3 mentions) Citric acid, by Merck Group (3 mentions) Disteroylphosphatidylcholine dspc, by Lipoid (2 mentions) Distearoylphosphatidylcholine dspc, by Lipoid (2 mentions) Mir nc, by Thermo Fisher Scientific (2 mentions) Sodium borate, by Merck Group (1 mentions) 2 iminothiolane, by Merck Group (1 mentions) 1 2 distearoyl sn glycero 3 phosphoethanolamine n, by Avanti Polar Lipids (1 mentions) 1 2 dioleoyl sn glycero 3 phosphoethanolamine n carboxyfluorescein, by Avanti Polar Lipids (1 mentions) Mir 34a, by Thermo Fisher Scientific (1 mentions)

4 protocols using peg2000 cer16

Lipid-based Anti-microRNA103/107 Delivery

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-microRNA103/107 (5′-rUrCrArUrArGrCrCrCrUrGrUrArCrArArUrGrCrUrGrCrU-3′) and (5′-/5RhoR-XN/rUrCrArUrArGrCrCrCrUrGrUrArCrArArUrGrCrUrGrCrU-3′), namely anti-miR103/107, was synthesized by Tema Ricerca s.r.l. (Bologna, Italy). 1,2-dioleyl-3-dimethylammonium propane (DODAP), N-palmitoyl-sphingosine-1-succinyl[methoxy(polyethylene glycol)2000] (PEG2000-Cer16) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000]-Maleimide (DSPE-PEG-Mal) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) (ammonium salt) were obtained by Avanti Polar Lipids. Disteroylphosphatidylcholine (DSPC) was kindly offered from Lipoid GmbH (Cam, Switzerland). Cholesterol (CHOL), human transferrin (Tf), ammonium ferrithiocyanate, sodium borate, sodium chloride, sodium citrate, sodium phosphate, HEPES, ethylenediaminetetraacetic acid (EDTA), citric acid, Sepharose G-25, and 2-iminothiolane (Traut’s reagent) were purchased from Sigma-Aldrich (USA). Ethanol and other solvents were obtained by Exacta Optech (Italy).

Cepparulo P., Cuomo O., Campani V., Vinciguerra A., Sisalli M.J., Nele V., Anzilotti S., Valsecchi V., Casamassa A., Brancaccio P., Scorziello A., De Rosa G., Annunziato L, & Pignataro G. (2024). Anti-miRNA103/107 encapsulated in transferrin-conjugated lipid nanoparticles crosses blood-brain barrier and reduces brain ischemic damage. Molecular Therapy. Nucleic Acids, 35(1), 102131.

+ Open protocol

+ Expand

Lipid Nanoparticle Formulation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

1,2-dioleyl-3-dimethylammonium propane (DODAP) and N-palmitoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)2000]} (PEG₂₀₀₀-Cer₁₆) were purchased by Avanti Polar Lipids (Darmstadt, Germany). Distearoylphosphatidylcholine (DSPC) was kindly offered from Lipoid GmbH (Cam, Switzerland). Cholesterol (CHOL), sodium chloride, sodium phosphate, HEPES, citric acid and sodium citrate were purchased by Sigma-Aldrich (St. Louis, MO, USA). Ethanol and other solvents were obtained by Exacta Optech (Modena, Italy).

Fattore L., Campani V., Ruggiero C.F., Salvati V., Liguoro D., Scotti L., Botti G., Ascierto P.A., Mancini R., De Rosa G, & Ciliberto G. (2020). In Vitro Biophysical and Biological Characterization of Lipid Nanoparticles Co-Encapsulating Oncosuppressors miR-199b-5p and miR-204-5p as Potentiators of Target Therapy in Metastatic Melanoma. International Journal of Molecular Sciences, 21(6), 1930.

+ Open protocol

+ Expand

Lipid-Based Formulation for miR-34a Delivery

Check if the same lab product or an alternative is used in the 5 most similar protocols

1,2-dioleyl-3-dimethylammonium propane (DODAP) and N-palmitoyl-sphingosine-1-succinyl[methoxy(polyethylene glycol)2000] (PEG2000-Cer16) were purchased by Avanti Polar Lipids. Disteroylphosphatidylcholine (DSPC) was kindly offered from Lipoid GmbH (Cam, Switzerland). Cholesterol (CHOL), sodium chloride, sodium phosphate, HEPES, citric acid and sodium citrate was purchased by Sigma Aldrich (USA), ethanol and other reagents were obtained by Carlo Erba Reagenti (Italy). miR-34a were purchased by Life Technologies as ds-oligonucleotide with the sequence of miR-34a duplex as reported in miR.org database. As control an oligonucleotide with scrambled sequence (miR-NC) was used (Life Technologies).

Di Martino M.T., Campani V., Misso G., Gallo Cantafio M.E., Gullà A., Foresta U., Guzzi P.H., Castellano M., Grimaldi A., Gigantino V., Franco R., Lusa S., Cannataro M., Tagliaferri P., De Rosa G., Tassone P, & Caraglia M. (2014). In Vivo Activity of MiR-34a Mimics Delivered by Stable Nucleic Acid Lipid Particles (SNALPs) against Multiple Myeloma. PLoS ONE, 9(2), e90005.

+ Open protocol

+ Expand

Lipid-based miRNA Delivery System

Check if the same lab product or an alternative is used in the 5 most similar protocols

1,2-Dioleyl-3-dimethylammonium propane (DODAP) and N-palmitoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)2000]} (PEG₂₀₀₀-Cer₁₆) were purchased from Avanti Polar Lipids. Distearoylphosphatidylcholine (DSPC) was kindly offered from Lipoid GmbH (Cam, Switzerland). Cholesterol (CHOL), human transferrin (Tf), sodium chloride, sodium phosphate, HEPES, citric acid, and sodium citrate were purchased from Sigma Aldrich (USA). Ethanol and other solvents were obtained from Carlo Erba Reagenti (Italy). MiR-34a wild type sequence obtained by miR.org database (5′-UGG CAG UGU CUU AGC UGG UUG U-3′) was directly synthesized by MWG (Germany). As control an oligonucleotide with scrambled sequence (miR-NC) was used (Life Technologies).

Scognamiglio I., Di Martino M.T., Campani V., Virgilio A., Galeone A., Gullà A., Gallo Cantafio M.E., Misso G., Tagliaferri P., Tassone P., Caraglia M, & De Rosa G. (2014). Transferrin-Conjugated SNALPs Encapsulating 2′-O-Methylated miR-34a for the Treatment of Multiple Myeloma. BioMed Research International, 2014, 217365.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!