Experiments were performed using C57BL/6J mice (male, 3 weeks old) and C.B-17/IcrHsd-Prkdcscid mice (SCID, male, 6 weeks old) (both from Japan SLC, Inc., Hamamatsu, Japan). Animals were housed at a specific pathogen-free environment in the animal experiment facility of Matsumoto Dental University High-Tech Center. The animals were kept under controlled environmental conditions at 22°C and 50% humidity with a 12 h light/dark cycle, with free access to food and water. All procedures and experiments in this study were performed in accordance with the guidelines laid down by the National Institutes of Health (NIH) in the United States, regarding the care and use of animals for experimental procedures. This study was approved by the Matsumoto Dental University Committee on Intramural Animal Use (Nos. 289 and 364).
C b 17 icrhsd prkdcscid mice
Manufactured by Japan SLC
Sourced in Japan
The C.B-17/IcrHsd-Prkdcscid mice are a strain of immunodeficient mice. They have a mutation in the Prkdc gene, which results in a severe combined immunodeficiency (SCID) phenotype. These mice lack functional T and B cells, making them useful for various research applications where an immunodeficient model is required.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6j mice, by Japan SLC (1 mentions)
Somnosuite small animal anesthesia system, by Kent Scientific (1 mentions)
Pen strep, by Thermo Fisher Scientific (1 mentions)
Um uc 3, by Fujifilm (1 mentions)
Ht1376, by Fujifilm (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a medium, by Thermo Fisher Scientific (1 mentions)
Umuc3, by American Type Culture Collection (1 mentions)
Ht1376, by American Type Culture Collection (1 mentions)
4 protocols using c b 17 icrhsd prkdcscid mice
1
Mouse models for biomedical research
2
Immunodeficient Mouse Study Protocol
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In this experimental study, a total of 60 male immunodeficient
(C.B-17/IcrHsd-Prkdcscid) mice (weight, 24 (link)-26 g; age, eight weeks) were
purchased from Japan SLC, Inc. (Kyoto, Japan). All mice were housed in standard plastic
cages and provided food and water ad libitum at Kindai University at 24˚C with a 12 hours
light/ dark cycle. The experimental procedures for the mice were approved by the Kindai
University Animal Care and Use Committee (approval no. KAPR-2021-001), and the study was
performed in compliance with the ARRIVE guidelines (20 (link)). Mice were anesthetized by
inhalation of isoflurane (3% induction and 1% maintenance) using the SomnoSuite Small
Animal Anesthesia System (Kent Scientific Corporation, Torrington, CT, USA). Before the
experiment, the animals were acclimated to the facility for one week. After the completion
of the experiment, the animals were euthanized using carbon dioxide at a flow rate of 30%
of the chamber volume per minute in their home cages. Death was verified by the cessation
of respiratory and cardiovascular movements by observation at room air for at least 10
minutes.
(C.B-17/IcrHsd-Prkdcscid) mice (weight, 24 (link)-26 g; age, eight weeks) were
purchased from Japan SLC, Inc. (Kyoto, Japan). All mice were housed in standard plastic
cages and provided food and water ad libitum at Kindai University at 24˚C with a 12 hours
light/ dark cycle. The experimental procedures for the mice were approved by the Kindai
University Animal Care and Use Committee (approval no. KAPR-2021-001), and the study was
performed in compliance with the ARRIVE guidelines (20 (link)). Mice were anesthetized by
inhalation of isoflurane (3% induction and 1% maintenance) using the SomnoSuite Small
Animal Anesthesia System (Kent Scientific Corporation, Torrington, CT, USA). Before the
experiment, the animals were acclimated to the facility for one week. After the completion
of the experiment, the animals were euthanized using carbon dioxide at a flow rate of 30%
of the chamber volume per minute in their home cages. Death was verified by the cessation
of respiratory and cardiovascular movements by observation at room air for at least 10
minutes.
3
SCID Mice for Immunological Research
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C.B-17/IcrHsd-Prkdcscid mice (Japan SLC, Shizuoka, Japan)
were obtained at 6 weeks of age and maintained under specific pathogen-free conditions.
The mice were used for the experiments at 7 weeks of age. All experiments and procedures
for the care and treatment of the animals used in the present study were performed in
accordance with the requirements of the Juntendo University Animal Care and
Experimentation Committee (Experimental Protocol: No. 2017040; Juntendo University, Tokyo,
Japan) and the SLC Animal Care and Experimentation Committee (Experimental Protocols: No.
BT18098 and No. 18099).
were obtained at 6 weeks of age and maintained under specific pathogen-free conditions.
The mice were used for the experiments at 7 weeks of age. All experiments and procedures
for the care and treatment of the animals used in the present study were performed in
accordance with the requirements of the Juntendo University Animal Care and
Experimentation Committee (Experimental Protocol: No. 2017040; Juntendo University, Tokyo,
Japan) and the SLC Animal Care and Experimentation Committee (Experimental Protocols: No.
BT18098 and No. 18099).
4
Establishment of Bladder Cancer Cell Lines
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Human bladder cancer cell lines UM-UC-3, T24, HT1376, and 5637 were provided by American Type Culture Collection. UM-UC-3 and HT1376 lines were cultured in Eagle's minimum essential medium (EMEM; Wako), 5637 cells in RPMI1640 (Gibco, Thermo Fisher Scientific, Inc.), and T24 cells in McCoy's 5A medium (Gibco, Thermo Fisher Scientific, Inc.). In all the cultures, the medium was supplemented with streptomycin (100 mg/ml) and penicillin (100 units/ml; Pen strep; Gibco, Thermo Fisher Scientific) as well as with 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Tokyo, Japan). Cells were maintained in a humidified incubator with 5% CO2 at 37°C.
We purchased 7-week-old female C.B-17/IcrHsd-Prkdcscid mice from Japan SLC (Hamamatsu, Japan). These animals were transferred to a temperature-controlled (20–26°C) and humidity-controlled (40–60%) room with a 12-h light/12-h dark cycle during the experimental period. All animal experiments were approved by the FUJIFILM Animal Experimentation Committee.
We purchased 7-week-old female C.B-17/IcrHsd-Prkdcscid mice from Japan SLC (Hamamatsu, Japan). These animals were transferred to a temperature-controlled (20–26°C) and humidity-controlled (40–60%) room with a 12-h light/12-h dark cycle during the experimental period. All animal experiments were approved by the FUJIFILM Animal Experimentation Committee.
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