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Chitosan oligosaccharide

Manufactured by Sisco Research Laboratories
Sourced in India

Chitosan oligosaccharide is a type of polysaccharide derived from the natural polymer chitin. It is a water-soluble compound composed of short chains of glucosamine and N-acetylglucosamine units. Chitosan oligosaccharide serves as a functional ingredient with potential applications in various industries.

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3 protocols using chitosan oligosaccharide

1

Chitosan-based Antimicrobial Nanoparticles

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Luria Bertini (LB), TMG buffer (Tris hydrochloride, magnesium sulfate, gelatin buffer), Muller Hinton Agar (MHA), Agar powder (Bacteriological grade), all antibiotic discs, and media were supplied by Himedia, Maharashtra, India. Trehalose dihydrate, chitosan oligosaccharide (extra pure, 90% degree of deacetylation), and sodium tripolyphosphate (STPP) were purchased from Sisco Research Laboratories, Mumbai, India. Glycerol (molecular biology grade, extra pure), DiD (DiIC18(5); 1,1′-dioctadecyl-3,3,3′,3′- tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt) were purchased from, Thermo Fisher, Waltham, MA, USA. All other chemicals and reagents used in this study were molecular biology grade, bacteriological, and lab grade.
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2

Chitosan Oligosaccharide Synthesis Protocol

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Glacial acetic acid (Merck
99–100%), hydrochloric acid (Merck 99%), sodium hydroxide (Merck,
99%), sodium acetate (Merck), ethanol (Sigma-Aldrich, 99.80%), chitosan
oligosaccharide, (C12H24N2O9) n (Mw < 3000 Da, 87% DDA, Sisco
Research Laboratories Pvt. Ltd., Maharashtra, India), pyridine-2-carboxaldehyde,
2-acetyl pyridine (Sigma-Aldrich), copper(II) chloride (Merck), sodium
chloroacetate (Thermo Fisher Scientific), carbon disulphide (S D Fine-Chem
limited), hydrazine monohydrate (Thermo Fisher Scientific), acetone
(Thermo Fisher Scientific), ammonium hydroxide, methanol, and other
analytical grade reagents were used as available with no further purification.
Crab shells as chitin wastes were fetched from a local crab market
of Kathmandu, Nepal.
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3

Chitosan-Carboxy Methyl Cellulose Hydrogel Synthesis

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Chitosan Oligosaccharide and Carboxy Methyl Cellulose were obtained from Sisco Research Laboratories Pvt. Ltd., Maharashtra, India. Ethanol was obtained from Honyon International Inc., Hong Yang Chemical Corp., China. Glutaraldehyde (GA) (25%, for synthesis) was procured from Molychem, Mumbai, India, and Hydrochloric acid (35% pure) was obtained from Merck Specialities Private Limited Mumbai, India. Salicylic acid (SA) was obtained from New Shakti Pharmaceuticals, Kanpur, U.P. Sodium citrate were procured from Loba Chemie, Mumbai, India. Goat blood was obtained from the local Butcher shop. Double distilled water was used throughout the study.
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