Anti vrk3

The following monoclonal antibodies were purchased: anti-p-ERK (abcam); anti-GAPDH (Santa Cruz Biotechnology); anti-VHR (BD Biosciences); anti-HA (Roche); anti-NeuN (Merck Millipore); anti-GST, anti-cleaved caspase-3, anti-Bax, and anti-Bak (Cell Signaling Technology). The following polyclonal antibodies were also obtained: anti-PSD95 (abcam); anti-VRK3, anti-VHR, anti-p-ERK, anti-ERK, and anti-His tag (Cell Signaling Technology); anti-VRK3 (Atlas antibodies); anti-HA epitope (YPYDVPDYA; Bethyl Laboratories); anti-FLAG epitope (D-8), anti-Lamin B (C-20), anti-MAP2 (I-18), anti-CDK5 epitope (C-8), and anti-p35 epitope (C-19) (Santa Cruz Biotechnology). Phos-VRK3 at Ser 108 (pS108) polyclonal antibody was generated and purified from two rabbitts and two rats immunized with carrier protein-conjugated phosphopeptide, GSRPPTPKSPQKTRKSPQ (residues 99–117 of human VRK3; AnyGen, Gwangju, Korea).

The following monoclonal antibodies were purchased: anti-HSP70, anti-p-ERK, and anti-α-synuclein (Abcam); anti-GAPDH (Santa Cruz Biotechnology); anti-VHR (BD Biosciences); anti-HA (Roche); anti-Aβ (BioLegend); anti-NeuN (Merck Millipore); anti-HSP70, anti-GST, anti-cleaved caspase-3, anti-Bak, and anti-Bax (Cell Signaling Technology). The following polyclonal antibodies were also obtained: anti-PSD95 (Abcam); anti-HSP70, anti-VRK3, anti-VHR, anti-p-ERK, anti-ERK, and anti-His (Cell Signaling Technology); anti-VRK3 (Atlas antibodies); anti-HA (YPYDVPDYA; Bethyl Laboratories); anti-FLAG (D-8), anti-Lamin B, and anti-MAP2 (I-18) (Santa Cruz Biotechnology).