Midazolam

Antipyrine, cimetidine, clotrimazole, N-desmethyl-tamoxifen hydrochloride, furosemide, hydrochlorothiazide, ketoprofen, maleic acid, (+/−)-metoprolol-(+)-tartrate, (+/−)-norverapamil hydrochloride, piroxicam, tamoxifen, terbutaline hemisulfate and (+/−)-verapamil hydrochloride 99% were purchased from Sigma-Aldrich (St. Louis, MO, USA); (+/−)-propranolol hydrochloride and ranitidine hydrochloride were obtained from Alfa Aesar GmbH & Co KG (Karlsruhe, Germany), and carbamazepine was purchased from Acros Organics (New Jersey, USA). Midazolam and α-hydroxyMidazolam were purchased from Lipomed AG (Arlesheim, Switzerland), and agar, calcium chloride dihydrate, glucose hydrate, magnesium chloride hexahydrate, potassium chloride, sodium chloride, sodium hydroxide, sodium phosphate monobasic and sodium hydrogencarbonate were obtained from Hänseler AG (Herisau, Switzerland). Sodium taurocholate was purchased from Prodotti Chimici e Alimentari S.p.A., (Basaluzzo, Italy) and lecithin (grade EPCS > 98% phospholipids) was obtained from Lipoid GmbH (Ludwigshafen, Germany).

Albendazole, 1-OH-albendazole, benzydamine, phenacetin, acetaminophen, bufuralol, flutamide, diclofenac, and paclitaxel were obtained from the AstraZeneca compound library. 2-OH-bupropion, 1-OH-bufuralol, 1-OH-midazolam, 4-OH-midazolam, 4-OH-mephenytoin, 4-OH-diclofenac, 2-OH-flutamide, benzydamine N-oxide, 4-nitrocatechol, β-Nicotinamide adenine dinucleotide hydrate (NADPH) and DMSO were obtained from Sigma (St. Louis, MO, USA). Bupropion was obtained from Kemprotec Limited (Smailthorn, Carnforth, United Kingdom), midazolam from Lipomed (Cambridge, MA, USA), 6α-OH-paclitaxel from EMD Chemicals (San Diego, CA, USA), and S-mephenytoin from Toronto Research Chemicals (Toronto, ON, Canada). P-nitrophenol and potassium dihydrogen phosphate were obtained from Thermo Fisher Scientific (Gothenburg, Sweden) and dipotassium hydrogen phosphate and magnesium chloride were obtained from Merck (Kenilworth, NJ, USA). Formic acid (98–100%, analytical grade) was obtained from Merck (Darmstadt, Germany), acetonitrile from Rathburn (Walkerburn, UK), and ethanol from CCS Healthcare AB, Borlänge, Sweden. Deionized water was produced in an ELGA purification system (High Wycombe, UK).