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3 protocols using sodium hydrogen carbonate

1

Analysis of Biogenic Amines by HPLC

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Sodium hydroxide, sodium hydrogen carbonate, ammonium hydroxide, and perchloric acid were purchased from Junsei Chemicals (Seoul, Korea). Dansyl chloride and standard BAs were purchased from Sigma-Aldrich (St. Louis, MO, USA). High-performance liquid chromatography (HPLC)-grade ammonium acetate and acetonitrile were purchased from Merck (Damstadt, Germany). All chemicals used in this study were of analytical grade and were used as supplied.
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2

Microbiological Analysis of Food and Beverages

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All chemicals used were of analytical grade. Potassium chromate, 0.1 N Sodium hydroxide, silver nitrate, methyl alcohol, ethyl alcohol, and sodium chloride were obtained from Duksan Pure Chemicals (Ansan, Gyeonggi-do, Korea). Sodium hydroxide, sodium hydrogen carbonate, and ammonium hydroxide were purchased from Junsei Chemicals (Tokyo, Japan). Formalin solution, standard methanol, ethanol, pentanol, propanol, and butanol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Plate count agar, nutrient agar, potato dextrose agar (PDA), and potato dextrose broth were purchased from Difco (Becton, Dickinson and Company, Sparks, MD, USA). Mannitol egg yolk polymyxin agar (MYP), egg yolk emulsion, and polymyxin B supplement were purchased from Oxoid LTD (Basingstoke, Hampshire, UK). 3M Yeast and Mold Petrifilm was purchased from 3M Health Care (St. Paul, MN, USA). API 50CHB and API 20E were obtained from bioMerieux (Marcy I’Etoile, France).
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3

Biogenic Amine Analysis by HPLC

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All chemicals and solvents were of the analytical and chromatographic grade. Standard biogenic amines (agmatine sulfate, tryptamine hydrochloride, 2-phenylethylamine, putrescine dihydrochloride, cadaverine dihydrochloride, histamine dihydrochloride, tyramine hydrochloride, spermidine trihydrochloride, and spermine tetrahydrochloride), dansyl chloride, and acetone were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium hydroxide, sodium hydrogen carbonate, ammonium hydroxide, and perchloric acid were purchased from Junsei Chemicals (Tokyo, Japan). High-performance liquid chromatography (HPLC) grade acetonitrile and ammonium acetate were obtained from Merck (Darmstadt, Germany). Nutrient agar was purchased from Difco (Sparks, MD, USA). API kits were purchased from BioMerieux (Marcy I’ Etoile, France).
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