Cytotoxicity assay was carried out using WST-1 cell viability assay kit (Roche Molecular Biochemicals, Indianapolis, IN, USA) as per the manufacturer’s instructions. Cells were plated in triplicate in 96-well plates at the density of 2.0 × 104 cells/well in CS-C medium. Following overnight culture, the cells were incubated with the indicated concentrations of SWCNT for 24 and 72 h. After incubation, WST-1 reagent was added and the cells were incubated for an additional 4 h. The plates were then read at the wavelength of 420 nm using a microplate reader (Model 3550; BioRad, Richmond, CA, USA).
Wst 1 cell viability assay kit
Manufactured by Roche
Sourced in United States, Germany
The WST-1 cell viability assay kit is a colorimetric assay that measures the metabolic activity of cells. The assay is based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells, resulting in the formation of a colored formazan dye that can be measured spectrophotometrically.
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Lab products found in correlation
2 protocols using wst 1 cell viability assay kit
1
Cytotoxicity Assay of SWCNT
2
Betulinic Acid Cytotoxicity Evaluation
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Betulinic acid was purchased form Sigma Aldrich (St Louis, MO, USA). The stock solution was prepared by diluting with DMSO (dimethyl sulfoxide) to nal concentration of 100 µM and stored at -20 o C until being used. Cell lines and all cell culture reagents were purchased from ATCC (American Type Culture Collection). WST-1 cell viability assay kit was obtained from Roche Life Science (Mannheim, Germany).
RNA isolation and cDNA synthesis kits were purchased from Thermo sher Scienti c (Massachusetts, USA). SNAIL-1 and Syndecan-2 ELISA kits were purchased form Abbkine Scienti c (California, USA). Migration and Invasion assay kits were bought from Trevigen Inc. (Gaithersburg, Maryland, USA).
Cell Lines and Cell Culture CAKI-2 (clear cell renal cell carcinoma) and ACHN (metastatic renal adenocarcinoma) cells were cultured in McCoy's 5A medium and EMEM (Eagle's Minimum Essential Medium), respectively. The cell culture media are supplemented with %1 Penicillin-Streptomycin and %10 Fetal Bovine Serum (FBS). All cell lines were maintained in humidi ed incubator with 5% CO 2 at 37°C.
RNA isolation and cDNA synthesis kits were purchased from Thermo sher Scienti c (Massachusetts, USA). SNAIL-1 and Syndecan-2 ELISA kits were purchased form Abbkine Scienti c (California, USA). Migration and Invasion assay kits were bought from Trevigen Inc. (Gaithersburg, Maryland, USA).
Cell Lines and Cell Culture CAKI-2 (clear cell renal cell carcinoma) and ACHN (metastatic renal adenocarcinoma) cells were cultured in McCoy's 5A medium and EMEM (Eagle's Minimum Essential Medium), respectively. The cell culture media are supplemented with %1 Penicillin-Streptomycin and %10 Fetal Bovine Serum (FBS). All cell lines were maintained in humidi ed incubator with 5% CO 2 at 37°C.
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