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2 protocols using cytokinin standards

1

Cytokinin Analysis of Psychotria kirkii

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H 2 O and acetonitrile (ULC/MS grade) were obtained from BioSolve (Valkenswaard, the Netherlands). Formic acid (99%, for analysis) was obtained from Acros Organics (Pittsburgh, PA). Cytokinin standards were acquired from Olchemim Ltd (Olomouc, Czech Republic). Preparation of Psychotria kirkii samples was performed according to the protocol described in Prinsen et al. (1998) [18] .
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2

Cytokinin-based Cell Viability Assay

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Cytokinin standards were obtained from OlChemIm (Olomouc, Czech Republic). Calcein AM (1 mg/mL solution), an LDH release kit and a Neurite outgrowth kit were purchased from ThermoFisher. Propidium iodide, dihydroethidium, components of caspase-3/7 assay buffer, DMEM/F12 1:1 medium, fetal bovine serum, trypsin, ATRA, salsolinol hydrobromide, glutamate monosodium salt, deferoxamine, N-acetylcysteine, Ac-DEVD-CHO, and DMSO for cell cultures were purchased from Sigma Aldrich (Merck). Ac-DEVD-AMC substrate was supplied by Enzo Life Science.
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