Total RNA were firstly extracted from HepG2 cells using Trizol reagent (Invitrogen, USA) and were then used to synthesize cDNA by reverse transcription kit (Axygen, USA). Real-time PCR was then performed to detect the expression of target genes using specific primers (Table 1 ) under the following conditions: 90°C denature for 30 s, 58°C annealing for 50 s, and 72°C elongation for 35 s, repeated for 35 cycles. On a fluorescent quantitative PCR platform, CT values of both target genes and GAPDH were collected for quantitative analysis according to 2−ΔCt method.
Reverse transcription kit
Manufactured by Corning
Sourced in United States, China
The Reverse Transcription Kit is a laboratory tool designed for the conversion of RNA into complementary DNA (cDNA). This kit provides the necessary reagents and enzymes to facilitate this process, which is a crucial step in various molecular biology applications, such as gene expression analysis and viral detection.
Automatically generated - may contain errors
2 protocols using reverse transcription kit
1
Quantifying Gene Expression in HepG2 Cells
2
Stem Cell Culture and Characterization
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α-Minimum Essential Medium, high-sugar Dulbecco Modified Eagle Medium (H-DMEM), and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY). ASA VI was purchased from PuYi (Nanjing, Jiangsu, China) and fulvestrant (estrogen receptor antagonist) was purchased from MedChemExpress (Monmouth Junction, NJ). Anti-CD44-PE, anti-CD31-PE, anti-CD73-PE, and anti-CD45-PE were purchased from SinoBiological (Beijing, China), and the reverse transcription kit was purchased from Axygen (San Francisco, CA). The alkaline phosphatase (ALP) assay kit was purchased from Jiancheng (Nanjing, Jiangsu, China).
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