Fibrin glue

Manufactured by Baxter

Sourced in United States

Fibrin glue is a surgical adhesive product used in medical procedures. It is composed of fibrinogen and thrombin, which when combined, form a fibrin clot that can be used to seal wounds and facilitate tissue healing. The core function of fibrin glue is to provide a natural, biocompatible sealant for various surgical applications.

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15 protocols using fibrin glue

Biocompatible Cartilage Fusion Protocol

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DLLA-EG and LAP were synthesized using a protocol
developed in our lab.^{14 (link)} For infiltration,
grafts and host tissues were cultured in a 25% DLLA-EG HBSS solution
with 0.2% LAP for 5 min. Afterward, the implant was inserted into
the host tissue with an open space ranging from 0 to 1 mm in diameter.
The open space was filled with the DLLA-EG/LAP solution, and then
the whole construct was subject to illumination for 2 min. We first
used the DLLA-EG/LAP solution to fill the open space but without the
presoaking (infiltration) process. Then, we used fibrin glue (Tisseel,
Baxter Healthcare Corp, Deerfield, IL) to fill the open space (without
using PDLLA-PEG); these were the two controls. All cartilage explants
were cultured in chondrogenic medium (DMEM with 1% l-alanyl-l-glutamine (GlutaMAX), 55 mg/L sodium pyruvate, 1% antibiotic–antimycotic,
1% insulin transferrin-selenium (Invitrogen, Carlsbad, CA, USA), 10
ng/mL transforming growth factor-β3 (TGF-β3; PeproTech,
Rocky Hill, NJ, USA), 100 nM dexamethasone, 50 μM l-ascorbic acid 2-phosphate, and 23 μM l-proline).

Kuang B., Yang Y, & Lin H. (2019). Infiltration and In-Tissue Polymerization of Photocross-Linked Hydrogel for Effective Fixation of Implants into Cartilage—An In Vitro Study. ACS Omega, 4(20), 18540-18544.

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Sciatic Nerve Transection and Repair

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The sciatic nerve of the right hindlimb was exposed via a muscle-splitting technique. The sciatic nerve was sharply transected 3 mm proximal to the trifurcation. Immediately following nerve transection, the nerve was repaired using microsuture and fibrin glue (Tisseel, Baxter, Deerfield, IL, USA). After coaptation, the muscle and skin were closed appropriately. Animals were recovered on a heating pad, anesthesia was reversed with atipamezole hydrochloride (1 mg/kg), and animals were returned to the central animal housing facility within 12 hours for close monitoring and postoperative care.

Vannucci B., Santosa K.B., Keane A.M., Jablonka-Shariff A., Lu C.Y., Yan Y., MacEwan M, & Snyder-Warwick A.K. (2019). WHAT IS NORMAL? NEUROMUSCULAR JUNCTION REINNERVATION AFTER NERVE INJURY. Muscle & nerve, 60(5), 604-612.

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Amniotic Membrane Scaffolds for Tissue Engineering

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Human amniotic membranes (CellReGen, Salt
Lake City, UT) were used in all experiments. All donors were screened
for infectious diseases prior to the harvest of the membranes. All
samples were in sterile and dry condition. Poly-4-hydroxybutyrate
(Tepha Inc., Cambridge, MA), a fully biodegradable polymeric biomaterial,
was used to produce electrospun scaffolds, as described before.^{11 (link)} In short, P4HB was dissolved in chloroform and
dimethylformamide (9:1 vol/vol) to form a 10% (w/w) solution. Electrospinning
was performed with a custom-built rig. The polymer solution was supplied
from a syringe pump via a positively charged spinneret to a negatively
charged, adjustable speed rotating collector. AM was mounted on the
P4HB scaffold with fibrin glue (Tisseel, Baxter, Utrecht, The Netherlands)
using a spraying device. The fibrin glue was prepared in accordance
with the instructions for use.

Maljaars L., Gudde A., Oosthuysen A., Roovers J.P, & Guler Z. (2024). The Regenerative Capacity of Tissue-Engineered Amniotic Membranes. ACS Applied Bio Materials, 7(3), 1441-1448.

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Modified Middle Fossa Approach for Surgical Exposure

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A modified MFA was used in all ears. After an 8 cm skin incision running vertically from the tragus, a 4 by 4 cm bone flap was cut vertical to the external auditory canal. The dura mater was gently elevated from the middle fossa plate using blunt instruments pushing cottonoids forward and laterally. No retractor was used in order to minimize the extradural retraction of the temporal lobe. Bipolar coagulation induced retraction of the dura mater and improved exposure of the bony surface. CSF leak and bleeding were cautiously avoided thanks to the use of cottonoids and Surgicel^®. As much as possible, the SCD was sought only when a dry operative field free of blood was obtained in order to avoid suctioning in its vicinity. When identified, the SCD was immediately plugged with bone wax and then covered with bone paté. Finally, a fascia temporalis patch was draped on the petrous bone and secured with 2 ml of fibrin glue (Tisseel, Baxter, USA). The numerous tegmental dehiscences often observed in the roof of the petrous bone were addressed and closed during the same surgical procedure with bone paté and fascia. The bone flap was put back after the dura mater had been attached by two silk sutures. The patient stayed for at least 24 h in an ICU for neurological monitoring.

Thomeer H., Bonnard D., Castetbon V., Franco-Vidal V., Darrouzet P, & Darrouzet V. (2015). Long-term results of middle fossa plugging of superior semicircular canal dehiscences: clinically and instrumentally demonstrated efficiency in a retrospective series of 16 ears. European Archives of Oto-Rhino-Laryngology, 273, 1689-1696.

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Examining Gpr126 Signaling at NMJ after Nerve Injury

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To examine the role of Gpr126 signaling at the NMJ after nerve injury, we chose the spinal accessory nerve (SAN) innervating the sternomastoid (SM) muscle. Young adult mutant and CTR mice (3-4 months of age, both sexes) were anesthetized with ketamine (50 mg/kg BW) and dexmedetomidine (1 mg/kg BW) and placed supine under a surgical microscope. After the appropriate depth of anesthesia, the neck was carefully shaved and cleansed with betadine scrub and alcohol pads. A vertical skin incision was made from the chin to the superior aspect of the sternum, and salivary glands and fat were retracted to allow access to the SAN deep to the SM. The left SAN was cut just lateral to its point of entry into the muscle (~6 mm away from the muscle). Immediately following nerve transection, Seprafilm was placed under the cut nerve ends to prevent retraction, and the nerve was repaired using fibrin glue (Tisseel, Baxter, Deerfield, IL, USA). For all mice, the right SAN was exposed but not injured as a sham uninjured control. Incisions were sutured closed, and anesthesia was reversed with atipamezole hydrochloride (1 mg/kg). Mice were administered pain-reducing medication, and they were recovered on a heating pad and returned to the central animal housing facility within 12 hours of the procedure for close monitoring and postoperative care.

Jablonka-Shariff A., Lu C.Y., Campbell K., Monk K.R, & Snyder-Warwick A.K. (2019). Gpr126/Adgrg6 contributes to the terminal Schwann cell response at the NMJ following peripheral nerve injury. Glia, 68(6), 1182-1200.

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Multimodal MRI Scaffold Characterization

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Unseeded and seeded scaffolds were maintained in IMDM media and
placed in 0.2–0.7 mL Eppendorf tubes. ¹H and ¹⁹F MRI/MRS were then conducted. For postmortem studies, ¹H/¹⁹F MRI measurements were performed on hearts
with control (unseeded) and labeled (seeded) scaffolds positioned
using fibrin glue (Baxter, UK) on the anterior epicardial surfaces.

Constantinides C., Basnett P., Lukasiewicz B., Carnicer R., Swider E., Majid Q.A., Srinivas M., Carr C.A, & Roy I. (2018). In Vivo Tracking and 1H/19F Magnetic Resonance Imaging of Biodegradable Polyhydroxyalkanoate/Polycaprolactone Blend Scaffolds Seeded with Labeled Cardiac Stem Cells. ACS Applied Materials & Interfaces, 10(30), 25056-25068.

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Spinal Cord Injury Repair Strategies

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Four weeks after spinal cord transection, rats were randomly assigned to one of the five experimental groups. In Group 1 (control, n = 14), the surgical wound was reopened, and the dural sac was exposed without removing the scar. In Group 2 (n = 14), the dural sac was reopened in order to carefully remove the scar, leaving an ~6-mm gap. Then, two injections of 5 μl each of DMEM medium (GIBCO) containing 3 × 10⁴ BMSCs were injected parasagittally on each stump of the spinal cord with a 2 mm depth at the edge of both the rostral and caudal stumps. In Group 3 (n = 14), the spinal cord scar was removed as described for Group 2, and then, three to five PPN segments, each 6 mm long, were longitudinally implanted in the spinal cord gap. The implants were affixed with fibrin glue (BAXTER). In Group 4 (n = 14), the treatments described in Groups 2 and 3 were combined. Finally, in Group 5, the treatment described in Group 2 was used, with two BMSC injections administered in each stump, but adding 6 μl of ChABC (2 units/ml Seikagaku 100332; Associates of Cape Cod) to each injection (injecting a total volume of 11 μl) and with PPN implanted in the cord gap as described in Group 3.

Buzoianu-Anguiano V., Rivera-Osorio J., Orozco-Suárez S., Vega-García A., García-Vences E., Sánchez-Torres S., Jiménez-Estrada I., Guizar-Sahagún G., Mondragon-Caso J., Fernández-Valverde F., Madrazo I, & Grijalva I. (2020). Single vs. Combined Therapeutic Approaches in Rats With Chronic Spinal Cord Injury. Frontiers in Neurology, 11, 136.

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Mesenchymal Stem Cell Transplantation for Traumatic Brain Injury

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Adult female SD rats weighing 200–250 g were used for this experiment. All animals were anesthetized through intra-peritoneal injection with a solution of ketamine (50 mg/kg) and xylazine (10 mg/kg). During surgery, the body temperature was maintained at 37^oC with a warm pad. A piece of skull bone (7 mm x 7 mm, 1.6 mm posterior to the lambda and 2.0 mm right to the midline) was removed with a dental microdrill. TBI was induced by driving the 3 mm diameter tip of an electromagnetically controlled cortical impact (CCI) device at a rate of 3.5 m/s with 2.5 mm of compression. Within 1 hour, 2 million hypoxic (N = 30) or normoxic (N = 30) MSCs were topically applied to the exposed cerebral cortex and a thin layer of fibrin glue (TISSEEL Baxter, Round Lake, IL, USA) was applied to keep the cells in place and the skin was closed with 3-0 silk sutures. The same procedures were performed in the control rats, but they did not receive MSC transplantation or fibrin (N = 30). In total, 15 animals were used for sham surgery and underwent craniectomy without TBI.

Ma H., Lam P.K., Tong C.S., Lo K.K., Wong G.K, & Poon W.S. (2019). The neuroprotection of hypoxic adipose tissue-derived mesenchymal stem cells in experimental traumatic brain injury. Cell Transplantation, 28(7), 874-884.

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Adhesive Strength of Wound Sealants

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Wound closure adhesion testing was performed on the TA Instruments DMA Q800. 1 cm by 1 cm sections of porcine small intestine were attached to rectangular clamps using cyanoacrylate glue. Small intestine was warmed in ambient air to 37 °C before testing. The rectangular clamps were brought together end to end, and 1 mL of sealant polymer solution, 50 mL of cyanoacrylate glue, or 50 mL of fibrin glue (Tisseel, Baxter International Inc., Deerfield IL) was deposited on this joint, closing the gap between the two intestine-coated clamps (see ASTM F2458–05) [21 ]. The sealant was carefully applied and trimmed to avoid coating the interface between the ends and edges of the clamps. It was then allowed to set at 37 °C for 10 min before testing. A controlled force ramp was used to increase force at a rate of 1 N min⁻¹ until failure. Failure type was recorded as either adhesive or cohesive. Force values were normalized to the surface area of intestine coated by the adhesive, which was measured using calipers, giving adhesive strength. Each sample type was replicated five times (n= 5).

Daristotle J.L., Zaki S.T., Lau L.W., Torres L J.r., Zografos A., Srinivasan P., Ayyub O.B., Sandler A.D, & Kofinas P. (2019). Improving the adhesion, flexibility, and hemostatic efficacy of a sprayable polymer blend surgical sealant by incorporating silica particles. Acta biomaterialia, 90, 205-216.

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Autologous Limbal Stem Cell Transplant for Corneal Regeneration

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Patients were screened prior to surgery and were assigned a unique identification number. Screening included assessment of both ocular and systemic parameters. The fibrovascular pannus covering the cornea was removed with sharp and blunt dissection, and any bleeding vessels were cauterised with bipolar cautery. A sterile PLGA membrane was then secured in place on the denuded cornea using fibrin glue (Tisseel, Baxter India, India). A 2×2 mm strip of limbal tissue was taken from the healthy eye and divided into 8–10 pieces and distributed evenly over the PLGA membrane on the eye. Finally, a soft bandage contact lens (Johnson & Johnson Vision Care, Florida, USA) was placed on the eye. Patients were monitored on day 1 postsurgery in the hospital and then discharged. Patients were monitored for any adverse events at every follow-up visit using the procedures as listed in online supplemental table 1.

Ramachandran C., Deshpande P., Ortega I., Sefat F., McKean R., Srivastava M., MacNeil S., Basu S, & Sangwan V.S. (2021). Proof-of-concept study of electrospun PLGA membrane in the treatment of limbal stem cell deficiency. BMJ Open Ophthalmology, 6(1), e000762.

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