Legs were drawn on squared paper using a Reichert binocular microscope with an ocular screen. Details of the male and female genitalia were drawn by means of Abbe‘s drawing apparatus on a compound microscope (JENAVAL) at larger magnification (130–500×). Wings were photographed on the same microscope with an attached digital camera (Nikon COOLPIX 4500). Whole specimens were photographed by means of digital camera Canon EOS 5D Mark III with macro lens Canon MP-E 65 mm 1–5× and ring macro flash Canon MR-14EX.
Mp e 65 mm 1 5
Manufactured by Canon
The Canon MP-E 65 mm 1–5× is a specialized macro lens designed for close-up photography. It offers a magnification range of 1× to 5×, allowing for high-resolution capture of small subjects. The lens features a fixed focal length of 65 mm and a maximum aperture of f/2.8.
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4 protocols using mp e 65 mm 1 5
1
Detailed Anatomical Documentation of Insect Specimens
2
Photographic Documentation of an Insect
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The only specimen examined was found when searching for insects sitting on a wall of a house in the Morávka village (Fig. 2) in the Moravskoslezské Beskydy Mts in the NE part of the Czech Republic (Fig. 1). The specimen was photographed alive (see Figs 3,6) by means of a digital camera Canon EOS 450D with a macro lens Canon MP-E 65 mm 1-5× and ring macro flash Canon MR-14EX. Because the insect moved rapidly is was immediately captured for further study. The specimen was caught into a vial and killed by ethyl acetate. The dead dry specimen was subsequently photographed by means of a modified ore microscope with an attached body of Canon EOS 450D. It was manually repositioned upwards between each exposure and the final photographs were compiled from multiple layers (30-35) using Helicon Focus 7. The obtained images were edited in Adobe Photoshop CS6. After photography the specimen was put into a vial with 70% ethanol. Identification was done by the first author. The voucher specimen is deposited in SMOC -Slezské zemské muzeum, Opava, Czech Republic.
Abbreviations: AOL -minimum distance between posterior ocellus and anterior ocellus; F1, F2, etc. -funicle segments one, two, etc.; FV -minimum width of frontovertex; POL -minimum distance between posterior ocelli.
Abbreviations: AOL -minimum distance between posterior ocellus and anterior ocellus; F1, F2, etc. -funicle segments one, two, etc.; FV -minimum width of frontovertex; POL -minimum distance between posterior ocelli.
3
Morphological Analysis of Rhabdomastix
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The morphological terminology adopted here essentially follows McAlpine (1981) . Designation of the wing veins is given in Fig. 1 , and some special parts of the female terminalia of Rhabdomastix 3 (cf. also figures in Starý 2003 , 2004 ). Female terminalia were prepared by boiling in a solution of 10% KOH and preserved in glycerine in a small plastic tube pinned below the associated specimen. Line drawings were made using a drawing tube (camera lucida) attached to a compound microscope. Measurements were made using an ocular grid. Live specimens were photographed in special boxes by a digital camera (Canon EOS 60D) with a macro lens (Canon MP-E 65 mm 1–5×) and a ring macro flash (Canon MR-14EX).
4
Cross-polarized Microscopy of Specimens
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The digital images of the specimens were captured with a Canon EOS 5D SR camera mounted with Caron MP-E 65 mm (1–5×) or Canon EF 100-mm macro lenses under cross-polarised light, and were processed in Adobe Photoshop CC 2018. All measurements were processed with ImageJ version 1.49.
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