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Abl8197

Manufactured by Abcam
Sourced in United Kingdom

Abl8197 is a lab equipment product from Abcam. It is a device used for the analysis and measurement of various samples and materials in a laboratory setting. The core function of Abl8197 is to perform specific tasks required for scientific research and investigation.

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2 protocols using abl8197

1

TMJ Histological Analysis in β-catenin Mice

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We dissected TMJs from β-catenin(ex3)Co12ER mice and Cre-negative control mice. Samples were fixed in 10 % formalin, decalcified, and embedded in paraffin. The condyles were sectioned into serial sections at 3 µm thick in an anterior-posterior direction. Cell proliferation was carried out using anti-PCNA antibody at the dilution of 1:200 (abl8197, ABCAM®, Cambridge, UK). Apoptosis assay was carried out using a TUNEL assay kit according to the manufacturer’s instructions (G3250, Promega, Madison, WI, USA).
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2

TMJ Tissue Analysis in β-cat(ex3) Mice

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We dissected TMJ tissues from β-cat(ex3) Agc1CreER mice and Cre-negative controls. Samples were fixed in 10% formalin, decalcified, and embedded in paraffin. The condyles were sectioned into serial sections at 3-μm-thick in an anterior–posterior direction. Cell proliferation was carried out using anti-PCNA and anti-Ki67 antibodies at the dilution of 1:200 (abl8197, ab16667, Abcam, Cambridge, UK) and 1:2 000, respectively, as previously described.16 (link) Apoptosis assay was carried out using a TUNEL Assay Kit according to the manufacturer’s instructions (G3250, Promega, Madison, WI, USA).
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