Cdcl2 2.5 h2o

Mice were divided into two groups of ten or eleven mice each. At -72, -48, and -24 hr, animals of the group-1 were administered at 24-hr intervals by s.c. injection with 50 mg/kg ZnSO 4 (Nacalai Tesque, Kyoto, Japan). Twentyfour hours after the final ZnSO 4 injection, both group-1 and group-2 (not treated with ZnSO 4 ) were injected i.p. with 4 g/kg (at 5 mL/kg) CCl 4 . At 6 hr after the CCl 4 injection, three randomly selected mice from each group were euthanized and bled for plasma. The resulting plasma samples were stored at -80°C (pending alanine aminotransferase (ALT) assays). Livers were harvested from each of these animals, and separate samples from each liver were stored at -80°C (pending quantitative reverse transcription (RT)-PCR assay) or fixed in 15% neutral buffered formalin (pH 7.2) (pending processing for histological evaluation). The remaining seven or eight mice of each group were monitored every 3 hr until 24 hr after CCl 4 administration to determine acute mortality; the surviving animals were weighed and then terminated. Using equivalent protocols, protection from CCl 4 lethal toxicity was assessed using single pre-CCl 4 injections with 50 mg/kg ZnSO 4 or with 3 mg/kg CdCl 2 · 2.5H 2 O (Wako Chemical) administered s.c..