Anti β5

The following antibodies against human were used for Western blotting and immunoprecipitation technic: Anti-GAPDH (Sigma), anti-phospho-SRC Tyr416 and anti-SRC (Cell Signaling), anti-FGF-2 (Thermo Fisher Scientific Inc.), anti-FGFR1 and anti-β₅ (Cell Signaling), all at 1/1000 dilution. Secondary antibodies used were: anti-rabbit-HRP and anti-mouse-HRP (Dako), both at 1/1000 dilution.
For FACS analysis, anti-α_v-PE and anti-β₅ (Biolegend), anti-β₃-PE and anti-α_vβ₃-PE (BD Biosciences), anti-β₆ (clone IC8C3, Dr. D. Sheppard, UCSF, San Francisco) were used at 1/100 dilution. Anti-α_vβ₅ (clone P5H9, R&D Systems) and anti-α_vβ₆ (clone 10D5, Merck Millipore) were used for FACS at 1/50 dilution, and for function blocking experiments at 10 μg/ml, as well as anti-FGF-2 (R&D Systems) and anti-β₁ (clone Lia1/2, Beckman). Dead Cell Apoptosis Kit for PI-Annexin V staining was from Life technologies.
anti-phospho-SRC Tyr416 (Cell Signaling), anti-FGFR1 (Cell Signaling), anti-Phalloïdin-AlexaFluor 546 (Invitrogen), anti-FGF-2 (Thermo Fisher Scientific Inc.), as well as anti-αSMA (Sigma), anti-pan-Cytokeratin (Dako) and DAPI ProLong Gold mounting medium (Invitrogen) were used for immunofluorescent staining and immunohistochemistry.

Antibodies (Abs) anti-P4D1 ubiquitin, -β2, -PSMD2, -p62 were purchased from Santa Cruz Biotechnology (CA). Abs anti-α6 and -PSMD3 were obtained from Invitrogen. Anti-β-actin Ab was from Abgent. Anti-annexin 5 Ab was from BD Science. Abs anti-β1, -RPN10 and -RPT4 were from Enzo Life Science. Anti-β5, -PSMA2, -LC3B, -ADRM1, -AMBRA1 and -HYOU1 were from Cell Signaling Technology (Danvers, MA, USA). Abs anti-mTOR and -S100A9 were from Sigma (St. Louis, MO, USA). Abs anti-UVRAG, -TOLLIP, -VPS33A, and -VTI1A were from Thermo Fisher Scientific (Waltham, MA, USA). Donkey anti-rabbit TexRed and donkey anti-mouse FITC secondary Abs were from Jackson Immunoresearch. Goat anti-mouse Star 635 was from Abberior and Donkey anti-Rabbit Alexa Fluor 594 was from Invitrogen. Fluorescent secondary Abs IRDye^® 800CW and IRDye^® 680RD were from LICOR. Peroxidase Goat Anti-Rabbit IgG and Peroxidase Rabbit Anti-Mouse IgG were from Jackson Immunoresearch.