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T24 bladder cancer cells

The T24 bladder cancer cells are a well-characterized human urothelial carcinoma cell line. They are derived from the transitional cell carcinoma of the urinary bladder and are commonly used in cancer research.

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2 protocols using t24 bladder cancer cells

1

Prostate and Bladder Cancer Cell Culture

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VCaP, LNCaP, and NCI‐H660 prostate cancer cells, T24 bladder cancer cells, McCoy's 5 A Modified Medium, and fetal bovine serum (FBS) were purchased from ATCC (Manassas, VA). DMEM and RPMI‐1640 cell culture media and glutamine were purchased from Life technologies (Carlsband, CA). VCaP cells were maintained in DMEM supplemented with 10% FBS. LNCaP cells were maintained in RPMI‐1640 supplemented with 10% FBS, 2.8 mM lglutamine. NCI‐H660 cells were maintained in RPMI‐1640 medium supplemented with 5% FBS, 4 mM lglutamine, 0.005 mg/ml insulin, 0.01 mg/ml transferrin, 30 nM sodium selenite, 10 nM β‐estradiol, and 10 nM hydrocortisone. T24 bladder cancer cells were maintained in McCoy's 5 A Modified Medium, supplemented with 5% FBS. All cells were cultured in a 5% CO2 humidified incubator at 37°C.
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2

Culturing and Maintaining Prostate and Bladder Cancer Cell Lines

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VCaP, LNCaP, NCI-H660 prostate cancer cells, T24 bladder cancer cells, McCoy's 5A Modified Medium and fetal bovine serum (FBS) were purchased from ATCC (Manassas, VA). DMEM and RPMI-1640 cell culture media and glutamine were purchased from Life technologies (Carlsband, CA). VCaP cells were maintained in DMEM supplemented with 10% FBS. LNCaP cells were maintained in RPMI-1640 supplemented with 10% FBS, 2.8 mM L-glutamine. NCIH660 cells were maintained in RPMI-1640 medium supplemented with 5% FBS, 4 mM L-glutamine, 0.005 mg/mL insulin, 0.01 mg/mL transferrin, 30 nM sodium selenite, 10 nM beta-estradiol, and 10 nM hydrocortisone. T24 bladder cancer cells were maintained in McCoy's 5A Modified Medium, supplemented with 5% FBS. All cells were cultured in a 5% CO2 humidified incubator at 37°C.
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