Cells seeded on six-well plates at 1 × 106 cells and precultured were treated with 5 μg/mL of T-DM1 or HuIgG and incubated at 4 °C for 1 h. After changing the medium, cells were incubated at 37 °C for 0, 24, 48, or 72 h. The cells were collected and treated with PE Mouse Anti-Human IgG (BD Biosciences) and then incubated at 4 °C for 40 min. The samples were analyzed on the flow cytometer using FlowJo 10.4.1 (BD Biosciences).
Pe mouse anti human igg
Manufactured by BD
Sourced in United States
The PE Mouse Anti-Human IgG is a laboratory reagent used for the detection and analysis of human immunoglobulin G (IgG) in various applications. It consists of a fluorescent phycoerythrin (PE) label attached to a mouse-derived antibody specific for human IgG. This product can be utilized in techniques such as flow cytometry, immunohistochemistry, and immunoassays to identify and quantify the presence of human IgG in samples.
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2 protocols using pe mouse anti human igg
1
Quantification of T-DM1 Binding on Cells
2
Multiparametric Immune Cell Analysis
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PE mouse anti-human IgG and APC-H7 mouse anti-human CD3 were purchased from BD (Franklin Lakes, NJ, USA). Goat anti-human IgG1 PE was purchased from Thermo Fisher Scientific (Waltham, MA, USA). PE/Cyanine7 anti-human IFN-γ antibody, PE anti-human TNF-α antibody, APC anti-human CD107a (LAMP-1) antibody, APC anti-human CD56 (NCAM) antibody, PE anti-human CD16 antibody, PE anti-human CD335 (NKp46) antibody (9E2), Brilliant Violet 421™ anti-human CD45 antibody, PE/Cyanine7 anti-human CD14 antibody, PE anti-human CD19 antibody, and Zombie NIR™ Fixable Viability Kit were purchased from Biolegend (San Diego, CA, USA).
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