Log In Sign up
The largest database of trusted experimental protocols

Afdye 488 picolyl azide

Manufactured by Vector Laboratories
Visit Supplier

AFDye 488 picolyl azide is a fluorescent dye molecule designed for bioconjugation reactions. It contains an azido group for covalent labeling of biomolecules that have been modified with alkyne-containing moieties.

Automatically generated - may contain errors

Lab products found in correlation

5 ethynyl 2 deoxyuridine (edu), by Vector Laboratories (2 mentions) 5 ethynyl 2 deoxyuridine (edu), by Cayman Chemical (2 mentions) Afdye 647 picolyl azide, by Vector Laboratories (2 mentions)

Close spelling variants of this product

AFDye 488 Azide

2 protocols using afdye 488 picolyl azide

1

Quantifying DNA Synthesis Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
If measuring 5-ethynyl-2'-deoxyuridine (EdU) incorporation, cells were pulsed with 50 μM EdU (Cayman Chemical Cat# 20518) in growth media for 8 min prior to fixation and pre-extraction, unless otherwise stated. EdU is incorporated throughout the EdU pulse, such that incorporated EdU reflects the average rate of DNA synthesis over the length of the pulse. Thus an 8 min short EdU pulse is more reflective of the instantaneous DNA synthesis rate compared to a longer pulse such as 1 h. After blocking cells (prior to primary antibodies), cells were washed once with PBS, and then a click reaction67 (link) was performed in 2 mM CuSO4, 20 mg/mL sodium ascorbate in TBS (Tris 50 mM, NaCl 150 mM pH 8.3) with 3 μM AFDye 488 picolyl azide (Click Chemistry Tools Cat# 1276) or AFDye 647 picolyl azide (Click Chemistry Tools Cat# 1300) for 30 min, followed by a PBS wash.
Ratnayeke N., Baris Y., Chung M., Yeeles J.T, & Meyer T. (2023). CDT1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis. Molecular cell, 83(1), 26-42.e13.
+ Open protocol
+ Expand
2

Quantifying DNA Synthesis Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
If measuring 5-ethynyl-2'-deoxyuridine (EdU) incorporation, cells were pulsed with 50 μM EdU (Cayman Chemical Cat# 20518) in growth media for 8 min prior to fixation and pre-extraction, unless otherwise stated. EdU is incorporated throughout the EdU pulse, such that incorporated EdU reflects the average rate of DNA synthesis over the length of the pulse. Thus an 8 min short EdU pulse is more reflective of the instantaneous DNA synthesis rate compared to a longer pulse such as 1 h. After blocking cells (prior to primary antibodies), cells were washed once with PBS, and then a click reaction67 (link) was performed in 2 mM CuSO4, 20 mg/mL sodium ascorbate in TBS (Tris 50 mM, NaCl 150 mM pH 8.3) with 3 μM AFDye 488 picolyl azide (Click Chemistry Tools Cat# 1276) or AFDye 647 picolyl azide (Click Chemistry Tools Cat# 1300) for 30 min, followed by a PBS wash.
Ratnayeke N., Baris Y., Chung M., Yeeles J.T, & Meyer T. (2023). CDT1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis. Molecular cell, 83(1), 26-42.e13.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!