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Lcms grade solvents

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LCMS grade solvents are high-purity organic solvents specifically formulated for use in liquid chromatography-mass spectrometry (LC-MS) applications. These solvents are designed to meet the stringent requirements of LC-MS analysis, ensuring minimal interference and optimal performance in sensitive analytical techniques.

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Lab products found in correlation

Tripletof 5600 mass spectrometer, by AB Sciex (2 mentions) 425 system, by AB Sciex (2 mentions) 5 μm chromxp c18cl, by AB Sciex (2 mentions) 3 μm chromxp c18cl, by AB Sciex (2 mentions) Tryptone, by Carl Roth (1 mentions) Yeast extract, by Carl Roth (1 mentions)

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LCMS grade (8 citations)

3 protocols using lcms grade solvents

1

Peptide Separation and Analysis by LC-MS

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An Eksigent 425 system was coupled to an AB Sciex TripleTOF 5600+ mass spectrometer. The liquid chromatography system uses a trap column (ChromXP 5 μm ChromXP C18CL 120 Å 10×0.3mm) and an analytical column (Eksigent 3 μm ChromXP C18CL 120 Å 150×0.3mm). The analytical column temperature was maintained at 35 °C. LCMS grade solvents were purchased from Honeywell and composed of buffer A (0.1% formic acid in water (v/v)) and buffer B (0.1% formic acid in acetonitrile (v/v)). Peptides were reconstituted in 10μl 2% solvent B and 8μl was loaded at 10 μl/min for 3 minutes onto the trap column. Analytical separation was established by maintaining 3% B during loading. After a valve switch event, peptides were resolved using the analytical column at a flow rate of 5 μl/min with a linear gradient of 3–25% HPLC buffer B for 38 minutes, followed by a 5 min linear gradient to 32% B. Following the peptide elution window, in 2 min the gradient was increased to 80% B for 3 min. Initial chromatographic conditions were restored in 1 min and maintained for 8 min.
Edwinson A.L., Yang L., Peters S., Hanning N., Jeraldo P., Jagtap P., Simpson J.B., Yang T.Y., Kumar P., Mehta S., Nair A., Breen-Lyles M., Chikkamenahalli L., Graham R.P., De Winter B., Patel R., Dasari S., Kashyap P., Griffin T., Chen J., Farrugia G., Redinbo M.R, & Grover M. (2022). Gut Microbial β-Glucuronidases Regulate Host Luminal Proteases and are depleted in Irritable Bowel Syndrome. Nature microbiology, 7(5), 680-694.
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2

Cryopreservation and Cultivation of Propionibacterium freudenreichii

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Propionibacterium freudenreichii subsp. freudenreichii DSM 20271T (DSM 20271) was obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, Germany) and was kept as cryopreserved stock culture at −80°C in glycerol.
All chemicals were obtained from Sigma-Aldrich (Steinheim, Germany) unless otherwise specified. Tween 80 was purchased from GERBU Biotechnik GmbH (Heidelberg, Germany). [15N2]-ammonium sulphate [(15NH4)2SO4, 99%] was purchased from Eurisotop (Saarbrücken, Germany). Tryptone, yeast extract and agar were purchased from Roth (Karlsruhe, Germany). HPLC-UV grade solvents were obtained from VWR (Ismaning, Germany). LC-MS grade solvents were from Honeywell (Seelze, Germany).
Wang M., Asam S., Chen J., Ehrmann M, & Rychlik M. (2021). Production of Four 15N-Labelled Cobalamins via Biosynthesis Using Propionibacterium freudenreichii. Frontiers in Microbiology, 12, 713321.
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3

Peptide Separation and Analysis by LC-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols
An Eksigent 425 system was coupled to an AB Sciex TripleTOF 5600+ mass spectrometer. The liquid chromatography system uses a trap column (ChromXP 5 μm ChromXP C18CL 120 Å 10×0.3mm) and an analytical column (Eksigent 3 μm ChromXP C18CL 120 Å 150×0.3mm). The analytical column temperature was maintained at 35 °C. LCMS grade solvents were purchased from Honeywell and composed of buffer A (0.1% formic acid in water (v/v)) and buffer B (0.1% formic acid in acetonitrile (v/v)). Peptides were reconstituted in 10μl 2% solvent B and 8μl was loaded at 10 μl/min for 3 minutes onto the trap column. Analytical separation was established by maintaining 3% B during loading. After a valve switch event, peptides were resolved using the analytical column at a flow rate of 5 μl/min with a linear gradient of 3–25% HPLC buffer B for 38 minutes, followed by a 5 min linear gradient to 32% B. Following the peptide elution window, in 2 min the gradient was increased to 80% B for 3 min. Initial chromatographic conditions were restored in 1 min and maintained for 8 min.
Edwinson A.L., Yang L., Peters S., Hanning N., Jeraldo P., Jagtap P., Simpson J.B., Yang T.Y., Kumar P., Mehta S., Nair A., Breen-Lyles M., Chikkamenahalli L., Graham R.P., De Winter B., Patel R., Dasari S., Kashyap P., Griffin T., Chen J., Farrugia G., Redinbo M.R, & Grover M. (2022). Gut Microbial β-Glucuronidases Regulate Host Luminal Proteases and are depleted in Irritable Bowel Syndrome. Nature microbiology, 7(5), 680-694.
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