Cell counting kit 8 reagent

Manufactured by AbMole

Sourced in United States, China

The Cell Counting Kit‐8 Reagent is a colorimetric assay for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. The reagent contains a water‐soluble tetrazolium salt, which is reduced by dehydrogenases in living cells to produce a colored formazan dye. The amount of the formazan dye is directly proportional to the number of living cells, and can be measured by a spectrophotometer.

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Lab products found in correlation

Synergy h1 reader, by Agilent Technologies (1 mentions)

2 protocols using cell counting kit 8 reagent

Optimizing bEnd.3 Cell Viability

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bEnd.3 cells were treated with different concentrations of H₂O₂ (0, 500, 700, 800, 900 and 1000 µm) and NMN (0, 100, 200, 300, 500 and 600 µm) for 24 h. Then, bEnd.3 cells were treated with 100 µL of DMEM and 10 µL of Cell Counting Kit‐8 Reagent (AbMole BioScience, Inc., Houston, TX, USA). The plates were incubated in the dark for 1 h at 37°C. The optical density (OD) value was measured using a Synergy H1 Reader (BioTek, Winooski, VT, USA) at 450 nm.

Deng X., Liang X., Yang H., Huang Z., Huang X., Liang C., Kuang Y., Qin Y., Lin F, & Luo Z. (2021). Nicotinamide mononucleotide (NMN) protects bEnd.3 cells against H2O2‐induced damage via NAMPT and the NF‐κB p65 signalling pathway. FEBS Open Bio, 11(3), 866-879.

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Cell Proliferation Assay Protocol

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A total of 3 × 10³ cells were inoculated in a 96-well plate. After inoculation for 24 h/48 h/72 h/96 h, 10 μL of Cell Counting Kit-8 reagent (CCK-8; Abmole, Shanghai, China) was added to each well and incubated for 2 h at 37°C. The absorbance of each well was determined at 450 nm.

Zhao Y., Zhang X., Liu Y., Ma Y., Kong P., Bai T., Han M, & Li B. (2020). Inhibition of ALAS1 activity exerts anti-tumour effects on colorectal cancer in vitro. Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association, 26(3), 144-152.

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