For observation of Clr6-GFP, Sir2-GFP, cells were fixed with formaldehyde (final 3.2%) for 15 min at room temperature. For observation of Hst2-GFP, cells were fixed with 50% ethanol for 20 min at room temperature. Cells were washed with PBS and stained by DAPI. Imaging was conducted with EVOS FL Cell Imaging System (Thermo Fisher Scientific) equipped with UPlanSApo 100×/1.40 oil objective lens (Olympus). The signal at 530 nm wavelength was measured to detect the dead cells stained by Phloxine B.
Uplansapo 100 1.40 oil objective lens
Manufactured by Olympus
The UPlanSApo 100×/1.40 oil objective lens is an optical component designed for microscopy applications. It has a magnification of 100X and a numerical aperture of 1.40, making it suitable for high-resolution imaging. The lens is optimized for use with oil immersion, which can improve image quality and resolution. Detailed specifications and intended use are not provided.
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Lab products found in correlation
2 protocols using uplansapo 100 1.40 oil objective lens
1
Fluorescent Protein Imaging of Yeast Cells
2
Imaging One-Cell Embryos and Immunofluorescence
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Fluorescence images were obtained as described (Honda et al., 2017) , with an Orca-R2
digital CCD camera (Hamamatsu Photonics, Shizuoka, Japan) mounted on an IX71 microscope (Olympus, Tokyo, Japan) using a CSU-X1 spinning disc confocal system (Yokogawa Electric Corporation, Tokyo, Japan). MetaMorph software (Molecular Devices, California, USA) was used to control the microscopes. Live images of one-cell embryos were obtained using a UPlanSApo 60´/1.30 silicone oil objective lens (Olympus) with camera gain 255 and without binning, with 40-second intervals, 15 z-slices at intervals of 1 µm and a 300-ms exposure for GFP and 1000-ms exposure for mCherry. Immunofluorescence images were obtained using a UPlanSApo 100´/1.40 oil objective lens (Olympus) with camera gain 0 and without binning; with 20 z-slices at intervals of 0.5 µm and 250-ms exposure for Alexa488, 100-ms exposure for Alexa568 and 1000-ms exposure for DAPI. ImageJ/Fiji software (NIH; https://imagej.net/Fiji) was used for processing and analyzing the obtained images.
digital CCD camera (Hamamatsu Photonics, Shizuoka, Japan) mounted on an IX71 microscope (Olympus, Tokyo, Japan) using a CSU-X1 spinning disc confocal system (Yokogawa Electric Corporation, Tokyo, Japan). MetaMorph software (Molecular Devices, California, USA) was used to control the microscopes. Live images of one-cell embryos were obtained using a UPlanSApo 60´/1.30 silicone oil objective lens (Olympus) with camera gain 255 and without binning, with 40-second intervals, 15 z-slices at intervals of 1 µm and a 300-ms exposure for GFP and 1000-ms exposure for mCherry. Immunofluorescence images were obtained using a UPlanSApo 100´/1.40 oil objective lens (Olympus) with camera gain 0 and without binning; with 20 z-slices at intervals of 0.5 µm and 250-ms exposure for Alexa488, 100-ms exposure for Alexa568 and 1000-ms exposure for DAPI. ImageJ/Fiji software (NIH; https://imagej.net/Fiji) was used for processing and analyzing the obtained images.
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