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Anti cxcr4

Manufactured by Cell Signaling Technology
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Anti-CXCR4 is a primary antibody that specifically recognizes the CXCR4 protein. CXCR4 is a chemokine receptor that plays a key role in cell migration and signal transduction.

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Lab products found in correlation

Anti ccr5, by Cell Signaling Technology (1 mentions) Anti robo4, by Abcam (1 mentions) Slit2n, by Thermo Fisher Scientific (1 mentions) Anti phospho c src, by Cell Signaling Technology (1 mentions) Cocaine hydrochloride, by Merck Group (1 mentions) Anti phospho nf κb, by Cell Signaling Technology (1 mentions) Vegf c, by R&D Systems (1 mentions) Phospho perk, by Cell Signaling Technology (1 mentions) P gsk3β, by Cell Signaling Technology (1 mentions) P src, by Cell Signaling Technology (1 mentions) Twist, by Cell Signaling Technology (1 mentions) P akt, by Cell Signaling Technology (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Lysis buffer, by Cell Signaling Technology (1 mentions) Caspase 9, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) P paxillin, by Cell Signaling Technology (1 mentions) N cadherin, by Cell Signaling Technology (1 mentions)

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CXCR4 (9 citations)

2 protocols using anti cxcr4

1

HIV-1 gp120 Protein Inactivation and Signaling

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Recombinant HIV-1 gp120 protein was obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH (Germantown, MD). The experimental control of gp120 was prepared by boiling gp120 for 10 min to inactivate its protein activity while preserving its inherent endotoxin activity. Slit2N was purchased from PreproTech, Inc. (Rocky Hill, NJ). Cocaine hydrochloride (#C5776) was obtained from Sigma-Aldrich Corp. (St. Louis, MO). Double distilled water was the vehicle control used for Cocaine hydrochloride. Specific inhibitors for the dopamine-4 receptor (Sonepiprazole hydrate) and the sigma-1 receptor (Metaphit methanesulfonate salt) were purchased from Sigma-Aldrich. Anti-phospho-NFκB, anti-phospho-c-Src, anti-CCR5, anti-CXCR4, and anti-albumin antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA). Anti-phospho-paxillin, anti-D2-40, and anti-Robo4 antibodies were purchased from Abcam Inc. (Cambridge, MA). VEGF-C was purchased from R&D Systems, Inc. (Minneapolis, MN). All other antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).
Zhang X., Jiang S., Yu J., Kuzontkoski P.M, & Groopman J.E. (2015). Cocaine enhances HIV-1 gp120-induced lymphatic endothelial dysfunction in the lung. Physiological Reports, 3(8), e12482.
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2

Protein Expression Analysis in Cell Lysates

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Cells were harvested and lysed using lysis buffer (Cell Signaling Technology, Beverly, MA) supplemented with 5 mM NaF, 2 mM Na3VO4, 1 mM PMSF (polymethilsulfonyl fluoride), 5 μg/mL leupeptine, and 5 μg/mL aprotinin. Whole-cell lysates were subjected to sodium dodecyl sulfate-polyacrylamide gel eletrophoresis (SDS-PAGE) and transferred to polyvinyldene fluoride (PVDF) membrane (Bio-Rad Laboratories, Hercules, CA). The antibodies used for immunoblotting included: anti-CXCR4, -E-cadherin, -N-cadherin, -Slug, -Snail, -Twist, phospho(p)-ERK, -p-Akt, -p-paxillin, -p-Src, p-β-catenin, -β-catenin, -p-GSK3-β, -caspase-9, -PARP, -GAPDH, -tubulin, and -actin antibodies (Cell Signaling, Danvers, MA). Protein band quantification was obtained using Image J, as previously described. (Kovaleva et al., 2012 (link))
Roccaro A.M., Mishima Y., Sacco A., Moschetta M., Tai Y.T., Shi J., Zhang Y., Reagan M.R., Huynh D., Kawano Y., Sahin I., Chiarini M., Manier S., Cea M., Aljawai Y., Glavey S., Morgan E., Pan C., Michor F., Cardarelli P., Kuhne M, & Ghobrial I.M. (2015). CXCR4 regulates extra-medullary myeloma through epithelial-mesenchymal transition-like transcriptional activation. Cell reports, 12(4), 622-635.
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