Hypersil gold lc column

Sample separation was carried out using a Surveyor autosampler and an MSPumpPlus (Thermo Fisher Scientific, Runcorn, UK) LC system on a reverse phase Thermo Fisher Scientific Hypersil Gold LC column (1.0 × 100 mm, 5 μm) operated with a Hypersil guard cartridge, suitable for general separations. Mass spectrometry detection was performed using an LCQ ion trap (Thermo Fisher Scientific, Hemel Hempstead, UK) operated using an ESI source in positive mode. Instrument control and data analysis were carried out using Xcalibur 2.0.7 software with data processed using both the Quan Browser Xcalibur tool and Microsoft Excel.

HPLC analysis was carried out using an Agilent 1260 Infinity II HPLC system with a florescence detector set at λex = 330 nm and λem = 500 nm. A 150 × 4.6 mm, 3 μm Hypersil GOLD LC column (Thermo Fisher Scientific) was used isocratically with a (50/50 v/v) acetonitrile −10 mM phosphoric acid (pH = 2.5) mobile phase at a flow rate of 1 mL/min and a column oven temperature of 40 °C. Under these conditions, citrinin elutes with a retention time of approximately 3.6 min and a total run time of 6.0 min.