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L type hdl c

Manufactured by Fujifilm
Sourced in Japan

The L‐Type HDL‐C is a lab equipment product designed for the quantitative determination of high‐density lipoprotein cholesterol (HDL‐C) in human serum or plasma samples. It uses a direct enzymatic method to measure HDL‐C levels.

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2 protocols using l type hdl c

1

Metabolic Biomarker Quantification Protocol

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Plasma glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), non‐esterified free acid (NEFA), TG, total cholesterol, low‐density lipoprotein (LDL), high‐density lipoprotein (HDL) and glucose levels (Glu) were measured with L‐type Wako GOT‐J2, L‐type Wako GPT‐J2, NEFA‐HA Test Wako, L‐type Wako TG‐H, L‐type CHO H, L‐Type LDL‐C, L‐Type HDL‐C and L‐Type Glucose 2 (Wako Pure Chemical Industries, Osaka, Japan), respectively. 3‐Hydroxybutyrate was determined by Ketone test liquid (Sanwa Kagaku Kenkyusho, Nagoya, Japan) using auto analyser (7170, Hitachi Ltd., Tokyo, Japan). Plasma GIP, insulin, and adiponectin levels were measured using enzyme‐linked immunosorbent assay kits (LINCO RESEARCH, INC., Missouri, U.S.A., Shibayagi, Gumma, Japan, and Otsuka Pharmaceutical Ltd., Tokushima, Japan, respectively).
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2

Measurement of Plasma Lipids and Liver Lipids

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Plasma triglyceride, cholesterol (11488872216 and 11489232216, Roche diagnostics, Switzerland), high-density and low-density lipoprotein cholesterol (L-Type HDL-C and L-Type LDL-C, Wako Pure Chemicals Industries, Japan) concentrations were measured by enzymatic methods using the commercially available kit according to manufacturer’s instructions. Plasma creatine kinase (CK) activity was determined using Stanbio CK Liqui-UV® kit (2910-430, Stanbio Laboratory, USA). Total liver lipids were determined gravimetrically after extraction by the method of Bligh and Dyer24 (link). Individual hepatic lipids were quantified enzymatically (as described above) after solubilisation in isopropanol (Sigma-Aldrich, USA)25 (link).
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